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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Identification of chlorinated nitrobenzene residues in Mississippi River fish.

Residues of lower chlorinated nitrobenzenes have been found at levels up to about 1 ppm in 8 samples of Mississippi River fish. Electron capture gas chromatography (EC/GC) was used for determination after extraction and cleanup using a procedure based on the AOAC multiresidue method for organochlorine and organophosphorus pesticides in nonfatty foods. The residues found included 2-, 3-, and 4-chloronitrobenzene and 2,3- and 3,4-dichloronitrobenzene; identity was confirmed by GC/ mass spectrometry. GC retention times for 15 monochloro-through pentachloro-substituted nitrobenzene congeners were determined with OV-101 and mixed OV-101 + OV-210 columns at 130 degrees C. In studies of the nonfatty food extraction and cleanup procedures of the AOAC method, recoveries of 15 chlorinated nitrobenzenes from spiked fish samples ranged from 68 to 116%. GC of cleaned up fish extract aliquots equivalent to 20 mg sample allowed quantitation of individual congeners at levels of about 0.025 and 0.005 ppm with 3H and 63Ni EC detectors, respectively. The contamination of Mississippi River fish with chlorinated nitrobenzenes appears to be localized in a 150 mile section of the river extending from St. Louis, MO, to Cape Girardeau, MO; no chlorinated nitrobenzenes (less than 0.005 ppm) were detected in Mississippi River fish caught above or below this region of the river or in fish from the lower Missouri River, which joins the Mississippi River near St. Louis.[1]


  1. Identification of chlorinated nitrobenzene residues in Mississippi River fish. Yurawecz, M.P., Puma, B.J. Journal - Association of Official Analytical Chemists. (1983) [Pubmed]
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