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Kwan, T.K. et al.

Intermediates in 16-androstene biosynthesis in neonatal porcine testis.

The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3-week old piglets. Metabolites identified after derivatization by capillary gas-chromatography and further by combined gas chromatography-mass spectrometry were: 17-hydroxy- and 16 alpha-hydroxypregnenolone, dehydroepiandrosterone, 5-androstenediols, 5-pregnenediol and andien-beta. Pregnenolone and 16-dehydro-pregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas-chromatography.[1]

References

Intermediates in 16-androstene biosynthesis in neonatal porcine testis. Kwan, T.K., Honour, J.W., Taylor, N.F., Gower, D.B. FEBS Lett. (1984) [Pubmed]

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