Disease relevance of Androst-16-ene

• In incubations containing 5,16-androstadien-3-ol, mixed populations of Corynebacterium spp., isolated from the axilla, could generate many different 16-androstene metabolites, several of which were odorous [1].

High impact information on Androst-16-ene

• Intermediates in 16-androstene biosynthesis in neonatal porcine testis [2].
• Identification of 16-dehydropregnenolone as an intermediate in 16-androstene biosynthesis in neonatal porcine testicular microsomes [3].
• In pigs, a positive correlation has been found between cytochrome b5 (CYB5) and production of 16-androstene [4].
• Synthesis of free and sulphoconjugated 16-androstene steroids by the Leydig cells of the mature domestic boar [5].
• Leydig cells were purified from testes of mature male pigs and incubated with pregnenolone, or various individual 16-androstene steroids for 10 min, 1, 4 and 8h [5].

Biological context of Androst-16-ene

• Based on detection of these metabolites, a metabolic map for axillary corynebacterial 16-androstene biotransformations was proposed, detailing potential enzyme activities [1].
• Previous studies from this laboratory have suggested that the 3 beta-HSD-I reactions in the androgen and 16-androstene biosynthetic pathways may be catalysed by different enzymes with selective substrate specificities [3, 4] [6].
• The high concentration of 16-androstene steroids in boar glands was correlated with the presence of large amounts of binding protein for these steroids in the glands; smaller amounts of the binding protein were detected in female glands [7].

Anatomical context of Androst-16-ene

• It was found to be a nonglycosylated heterodimeric protein, belonging to the secretoglobin superfamily and the major 16-androstene-binding protein present in submaxillary salivary glands of the boar [8].
• Metabolism of the 16-androstene steroids in primary cultured porcine hepatocytes [9].
• The submaxillary glands of boars contain a low molecular mass protein, pheromaxein, which is capable of binding these 16-androstene pheromones [8].
• In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue [10].

Associations of Androst-16-ene with other chemical compounds

• Applications of gas chromatography-mass spectrometry in the study of androgen and odorous 16-androstene metabolism by human axillary bacteria [11].
• Studies of 16-androstene steroid biosynthesis and binding in human testis [12].
• The 16-androstene steroids, one of the principal causes of boar taint, are synthesized in the testis by the andien-beta synthase enzyme system [13].
• Two versions of a colorimetric assay for the androst-16-ene steroids were used, a complete version, in which cholesterol was removed from the tissue extracts using a digitonin-Sepharose affinity column, and a simplified version, which does not include this step [14].
• Samples of salivary gland and backfat were collected at slaughter for colorimetric assay of salivary and fat 16-androstene levels and fat skatole levels [15].

Gene context of Androst-16-ene

• Recent data has implicated an increased level of cytochrome b5 in the testes with the increased synthesis of the 16-androstene steroids [10].
• It was discovered that the enzyme responsible for the sulfoconjugation of the 16-androstene steroids is hydroxysteroid sulfotransferase [16].

References