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Affinity chromatography of acyl-CoA utilizing enzymes on Procion Red-agarose.

The dye Procion Red HE3B immobilized on agarose and available as Matrex Gel Red A is shown to bind citrate synthase and succinate thiokinase from a number of diverse organisms. Salt-gradient elution removes the enzymes in high yields and with substantial purification. The elution profiles follow a pattern similar to that of the molecular size variations of the enzymes.[1]

References

  1. Affinity chromatography of acyl-CoA utilizing enzymes on Procion Red-agarose. Weitzman, P.D., Ridley, J. Biochem. Biophys. Res. Commun. (1983) [Pubmed]
 
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