The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Effects of phospholipase C on the Na+-Ca2+ exchange and Ca2+ permeability of cardiac sarcolemmal vesicles.

We have examined the effects of phospholipase C pretreatment on Ca2+ transport in highly purified canine cardiac sarcolemmal vesicles. Na+-Ca2+ exchange, measured as Nai+-dependent Ca2+ uptake, is stimulated when 10-70% of the membrane phospholipid has been hydrolyzed. Although the phospholipase C treatment also increases sarcolemmal passive Ca2+ flux, the membrane maintains a sufficient permeability barrier for enhanced transport (via Na+-Ca2+ exchange) to be observed. These effects can be obtained with phospholipase C from either Clostridium perfringens or Bacillus cereus. We find that the phospholipase C (C. perfringens) preferentially hydrolyzes phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin leaving the negatively charged phospholipids, phosphatidylserine and phosphatidylinositol, intact. This suggests that the presence of negatively charged phospholipids is sufficient to ensure Na+-Ca2+ exchange activity. Thin section electron microscopy reveals gross morphological changes in phospholipase C-treated sarcolemma. The vesicles are aggregated and diacylglycerol droplets are visible. Fusion occurs such that adjacent vesicles share a common bilayer. It is surprising that vesicles displaying severe structural damage can demonstrate enhanced transport activity.[1]

References

 
WikiGenes - Universities