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Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C.

Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4 degrees C and high concentrations are shown to fuse completely to vesicles about 700-A diameter in 7 days, and these further fuse to about 950 A diameter vesicles after 3-4 weeks. The 950 A diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 months. The 950-A vesicles are characterized by agarose gel chromatography, freeze-fracturing electron microscopy, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.[1]


  1. Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C. Wong, M., Anthony, F.H., Tillack, T.W., Thompson, T.E. Biochemistry (1982) [Pubmed]
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