Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Das, M.

Initiation of nuclear DNA replication: evidence for formation of committed prereplicative cellular state.

This paper explores the transitional states that bridge the gap between nuclear quiescence and mitogenesis. It presents evidence for the formation of a committed but prereplicative state. Quiescent murine Swiss 3T3 cells were exposed to an external mitogenic stimulant (epidermal growth factor or excess serum) and simultaneously to a synchronizer which inhibits entry into the S phase. Thus, the cells were stimulated to synthesize DNA, but the normal replicative response to this stimulus was blocked. The block to DNA replication was removed at varying times after removal of the stimulant. Experiments were done to monitor the decay of commitment to DNA synthesis after removal of the external stimulant. This decay turned out to be a first-order process. The half-life (time required for loss of the commitment to DNA synthesis in half of the initially sensitized cells) was found to be approximately 5 hr. The same result was found whether total DNA synthesis or individually replicating cells were measured and was independent of the type of external growth stimulant or blocker used. These results point to the existence, on the mitogenic pathway, of a committed but prereplicative state. The committed state appears to represent a unit or global property of the whole cell rather than, for example, a critical concentration of some active inducer molecule because the latter would display multi-hit decay kinetics rather than the single-step lability actually observed.[1]

References

Initiation of nuclear DNA replication: evidence for formation of committed prereplicative cellular state. Das, M. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.