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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection.

A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.[1]

References

  1. Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection. Vermeulen, N.P., Teunissen, M.W., Breimer, D.D. J. Chromatogr. (1978) [Pubmed]
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