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Tsuchiya, T. et al.

Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli.

A strain of EScherichia coli was constructed containing a plasmid from the Clarke-Carbon collection that showed high levels of melibiose transport activity. Membranes from the plasmid-containing strain were extracted with octyl-beta-D-glucopyranoside and melibiose transport was reconstituted in liposomes. The proteoliposomes exhibited counterflow activity, as well as membrane potential and sodium gradient-driven melibiose accumulation.[1]

References

Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. Tsuchiya, T., Ottina, K., Moriyama, Y., Newman, M.J., Wilson, T.H. J. Biol. Chem. (1982) [Pubmed]

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