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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Solid-phase immunoassays for quantitation of antibody to bovine white matter proteolipid apoprotein.

Two solid-phase immunoassays have been developed for quantitation of antibodies to bovine white matter proteolipid apoprotein. Conditions were established for optimal specific antibody binding. Water-soluble proteolipid apoprotein was bound to microtiter plates and plates were incubated with test serum. Goat anti-rabbit IgG conjugated with horseradish peroxidase was used as the second antibody for an enzyme-linked immunospecific assay and 125I-labeled protein A for a radioimmunoassay. Both procedures have been used to follow the time course of anti-proteolipid antibody production in rabbits and to compare different immunization protocols.[1]

References

  1. Solid-phase immunoassays for quantitation of antibody to bovine white matter proteolipid apoprotein. Macklin, W.B., Lees, M.B. J. Neurochem. (1982) [Pubmed]
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