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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Entry of insulin into human cultured lymphocytes: electron microscope autoradiographic analysis.

Electron microscope autoradiographs were prepared of IM-9 human cultured lymphocytes incubated with iodine-125-labeled insulin. With the use of [125I]insulin and Ilford L-4 emulsion, the technique had a resolution half-distance of approximately 0.085 micrometer. Autoradiographs revealed a time-dependent entry of insulin into the cell interior that was maximal after 30 minutes of incubation. At this time point nearly 40 percent of the [125I]insulin was in the interior of the cell at a distance 1 micrometer or greater from the plasma membrane. Grain distribution and volume density analyses revealed that the intracellular insulin was concentrated in the endoplasmic reticulum and nuclear membrane.[1]

References

  1. Entry of insulin into human cultured lymphocytes: electron microscope autoradiographic analysis. Goldfine, I.D., Jones, A.L., Hradek, G.T., Wong, K.Y., Mooney, J.S. Science (1978) [Pubmed]
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