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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Interactions of cephalosporins and penicillins with nonpolar octadecylsilyl stationary phase.

The capacity factors of several penicillins and cephalosporins, as well as those of 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxycephalosporanic acid, were determined at pH 2.5-7.5 with different methanol concentrations in the mobile phase. The influence of ionic strength on activity factors also was studied. Some theoretical equations providing a quantitative description of the influence of the mobile phase pH on the retention of penicillins and cephalosporins by an octadecylsilyl stationary phase were established. The analysis of experimental data by a nonlinear least-squares fit to theoretically deduced equations permitted determination of the capacity factors of anionic, cationic, zwitterion, and undissociated forms of the substances studied.[1]

References

  1. Interactions of cephalosporins and penicillins with nonpolar octadecylsilyl stationary phase. Salto, F., Prieto, J.G., Alemany, M.T. Journal of pharmaceutical sciences. (1980) [Pubmed]
 
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