The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Effects and metabolic pathway of 4-dimethylaminophenol during kidney perfusion.

1. Isolated rat kidneys were perfused (single pass) with 4 to 40 microM soln. of 4-dimethylamino[14C]phenol (DMAP). 2. Nephrotoxicity was not detected at concn. of 14C-DMAP up to 18 microM; higher conc. resulted in irreversible loss of physiological functions with simultaneous five-fold increase in covalently bound 14C. 3. At all concn., 85% of the post-renal 14C was due to unchanged DMAP, while 15% corresponded to DMAP conjugates. These conjugates were identified as DMAP-glucuronide (90% total), DMAP-sulphate and DMAP-thioethers. 4. All DMAP conjugates were conc. in the urine with urine/perfusate concn. ratios in the range 5-7 for the glucuronide, 50-100 for the sulphate, and 10-20 for the thioethers. 5. Rates of formation fo metabolites vs DMAP concn. followed Michaelis-Menten kinetics for DMAP-sulphate (Km 25 microM, Vmax 2.2 nmol/min per g) and DMAP-thioethers (Km range 10-100 microM, Vmax 4 nmol/min per g). DMAP-glucuronide formation rate increased linearly with DMAP concn. and showed a four-fold increase at toxic DMAP doses. 6. From the data for DMAP conjugation capacity, urine/perfusate concn. ratios of DMAP conjugates and microautoradiography, it is suggested that DMAP conjugation is located mainly in the proximal convoluted tubule, where deterioration of renal functions originates.[1]

References

  1. Effects and metabolic pathway of 4-dimethylaminophenol during kidney perfusion. Elbers, R., Kampffmeyer, H.G., Rabes, H. Xenobiotica (1980) [Pubmed]
 
WikiGenes - Universities