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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene.

We cloned the full genomic DNA of yeast (Saccharomyces cerevisiae) O6-methylguanine-DNA methyltransferase (MGMT) gene and examined its expression. The expression of yeast MGMT gene is decreased when cells reach stationary phase and cannot be induced by the pretreatment with alkylating agents, methylmethanesulfonate (MMS) or N-methyl-N'-nitroso-N-nitrosoguanidine (MNNG). The transcription initiation site was determined by primer extension analysis. This analysis showed that the authentic start codon is the ATG at position +32 from transcription initiation site.[1]

References

  1. Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene. Joo, J.H., Rho, J.K., Kim, J.H., Kim, W.J., Choe, S.Y., Park, S.D. Cell. Mol. Biol. (Noisy-le-grand) (1995) [Pubmed]
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