Ras induces a general DNA demethylation activity in mouse embryonal P19 cells.
We demonstrate that expression of v-Ha-ras in mouse embryonal P19 cells results in genome-wide demethylation. Analysis of the pattern of methylation of specific genes reveals that different types of genes are demethylated in the ras transfectants: skeletal muscle specific genes, a gene specifically expressed in the adrenal cortex (c21), ubiquitous genes, and exogenously introduced sequences. Transient transfection and in vitro demethylation assays reveal that the ras transfectants express high levels of a general DNA demethylation activity. This demonstrates that the general DNA demethylation activity in mouse embryonal cells is controlled by an important cellular signal transducer and that DNA demethylase is a potential downstream effector of Ras.[1]References
- Ras induces a general DNA demethylation activity in mouse embryonal P19 cells. Szyf, M., Theberge, J., Bozovic, V. J. Biol. Chem. (1995) [Pubmed]
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