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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Anderson, M.C. et al.

Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding.

A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.[1]

References

Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding. Anderson, M.C., Tipton, K.F. J. Neural Transm. Suppl. (1994) [Pubmed]

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