Cloning and sequencing of rat liver carboxylesterase ES-3 (egasyn).
The cDNA of the rat carboxylesterase ES-3 encodes a polypeptide with 561 amino acid residues including a cleavable signal peptide at the N-terminus. The processed polypeptide shows over 90% sequence identity to mouse egasyn (ES-22); its calculated pI (5.5) matches the value determined for purified liver ES-3. The product expressed in COS cells migrates in native gels in the region of ES-3 and is similarly active on acetanilide. It is retained in the cells, as predicted from its C-terminus HTEL, and bears a single endo-H sensitive oligosaccharide chain. The nonglycosylated form expressed in the presence of tunicamycin is also intracellular, but substantially less active.[1]References
- Cloning and sequencing of rat liver carboxylesterase ES-3 (egasyn). Robbi, M., Beaufay, H. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
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