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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning of toxic genes with mini-mu derivative of bacteriophage mu.

We describe an in vivo cloning method using mini-Mu phage for genes, which cannot be cloned on multicopy vectors, mainly for their toxicity. We have successfully cloned succinate dehydrogenase ( sdh) gene E. coli which was inactivated with defined insertion of fragment Kmr by this method. The most of obtained Kmr clones of mini-Mu transductants have contained the sequence of whole sdh gene. The intact gene of sdh can be reconstructed by site-directed mutagenesis.[1]

References

  1. Cloning of toxic genes with mini-mu derivative of bacteriophage mu. Stuchlík, S., Janitorová, V., Turna, J. Acta Virol. (1993) [Pubmed]
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