The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

An activation-associated ganglioside in rat thymocytes.

During the activation of rat thymocytes elicited by stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophore A23187, the total amount of gangliosides per cell was demonstrated to increase, reaching a maximum level several times higher than that of resting cells at 48 h after activation when measured at 24-h intervals. The amount of the overwhelmingly predominant ganglioside in resting thymocytes, GD1c (NeuGc,NeuGc) (Nohara, K., Suzuki, M., Inagaki, F., and Kaya, K. (1991) J. Biochem. (Tokyo) 110, 274-278), was found to increase further as a result of activation. Furthermore, another ganglioside, which was barely recognizable in resting thymocytes, was found to increase in activated and proliferating thymocytes to a level similar to that of GD1c. This activation-associated ganglioside was isolated and its structure examined. On the basis of the results of compositional analysis, methylation analysis, sialidase hydrolysis followed by detection with cholera toxin B subunit on TLC, and proton NMR spectroscopy, this ganglioside was clarified to be a rare species of GD1b containing two N-glycolylneuraminic acid residues. On the other hand, when the thymocytes were activated using concanavalin A (ConA) as a stimulant, the amount of gangliosides per cell was increased more strikingly than that in thymocytes activated with TPA and A23187. In the ConA-activated thymocytes, many other gangliosides, in addition to GD1c and GD1b-(NeuGc,NeuGc), were demonstrated to appear in large amounts. The cause of this difference in gangliosides between thymocytes activated with a combination of TPA and A23187 and those activated with ConA is also discussed.[1]

References

  1. An activation-associated ganglioside in rat thymocytes. Nohara, K., Sano, T., Shiraishi, F. J. Biol. Chem. (1993) [Pubmed]
 
WikiGenes - Universities