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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.

The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.[1]

References

  1. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli. Ehrlich, S.D., Bursztyn-Pettegrew, H., Stroynowski, I., Lederberg, J. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
 
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