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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Molecular epidemiology of a parainfluenza type 3 virus outbreak on a pediatric ward.

Parainfluenza type 3 virus (PIV-3), an important cause of acute lower respiratory illness in children, can be transmitted nosocomially. To differentiate between nosocomial transmission and community-acquired infection, a polymerase chain reaction-based sequencing assay was developed for the 5' noncoding region of the PIV-3 fusion protein gene and was applied to virus specimens from 10 children infected with PIV-3 during a hospital outbreak. Four strains of PIV-3 were identified among the 10 virus isolates. Six isolates, which appeared to belong to 1 strain, were obtained from a cluster of nosocomial cases in a pediatric intermediate care unit. In contrast, the remaining 4 isolates, which appeared to belong to 3 different strains, were obtained from children infected in the community or elsewhere in the hospital. These data indicate that multiple strains of PIV-3 can be found during a single epidemic and provide evidence that infections within the intermediate care unit were probably caused by transmission of 1 strain of virus within the unit rather than reintroduction of virus by new patients or staff.[1]


  1. Molecular epidemiology of a parainfluenza type 3 virus outbreak on a pediatric ward. Karron, R.A., O'Brien, K.L., Froehlich, J.L., Brown, V.A. J. Infect. Dis. (1993) [Pubmed]
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