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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Functional expression of gastric H,K-ATPase using the baculovirus expression system.

A novel approach to construct a single recombinant baculovirus expressing two protein subunits simultaneously by replacing polyhedrin as well as p10 coding sequences is described. The recombinant baculovirus expressed the alpha- as well as the beta-subunit of the gastric H,K-ATPase. Sf9 cells infected with this virus exhibited a K(+)- and SCH 28080-sensitive ATP-dependent phosphorylation capacity in purified Sf9 membranes similar to native H,K-ATPase. This activity was not present in control membranes containing only one of the two H,K-ATPase subunits. We therefore conclude that both subunits are essential for the phosphorylation capacity of H,K-ATPase.[1]

References

  1. Functional expression of gastric H,K-ATPase using the baculovirus expression system. Klaassen, C.H., Van Uem, T.J., De Moel, M.P., De Caluwé, G.L., Swarts, H.G., De Pont, J.J. FEBS Lett. (1993) [Pubmed]
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