The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Both a ubiquitous factor mTEF-1 and a distinct muscle-specific factor bind to the M-CAT motif of the myosin heavy chain beta gene.

The A element, a fourteen base pair sequence in the rabbit myosin heavy chain (HC) beta promoter (-276/-263), contains the M-CAT motif, a cis-acting element found in several muscle-specific genes. The A element is essential for muscle-specific transcription of the myosin HC beta gene. Recently, we have identified both muscle-specific and ubiquitous factors (A1 and A2 factors, respectively) that bind to the A element. Since the sequence of the A element is very similar to the GTIIC motif in the SV40 enhancer, we examined the relationship between A-element-binding factors and a GTIIC binding factor TEF-1, recently isolated from HeLa cells. The GTIIC motif was bound by the A1 and A2 factors in muscle nuclear extracts and competed with the A element for DNA-protein complex formation. Antibody against human TEF-1 'supershifted' the ubiquitous A2 factor-DNA complex, but did not alter the mobility of the muscle-specific A1 factor-DNA complex. We isolated a murine cDNA clone (mTEF-1) from a cardiac cDNA library. The clone is highly homologous to Hela cell TEF-1. The in vitro transcription/translation product of mTEF-1 cDNA bound to the A element, and the DNA binding property of mTEF-1 was identical to that of the A2 factor. Transfection of mTEF-1 cDNA into muscle and non-muscle cells confirmed that mTEF-1 corresponds to A2, but not to A1 factors. The mTEF-1 mRNA is expressed abundantly in skeletal and cardiac muscles, kidney and lung, but it is also expressed at lower levels in other tissues. These results suggest that the M-CAT binding factors consist of two different factors; the ubiquitous A2 is encoded by mTEF-1, but the muscle-specific A1 factor is distinct from mTEF-1.[1]


WikiGenes - Universities