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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.

We report high-field continuous wave EPR spectra of P700+. in preparations obtained from deuterated cyanobacteria (Synechococcus lividus). Measurements were performed with photosystem I (PS-I) preparations, whole cells from cyanobacteria grown in 2H2O, and photosystem II (PS-II) preparations, as well as with protonated PS-I preparations. Because of the significantly improved resolution of our 140-GHz spectrometer (as compared with X- or Q-band EPR) the principal values of the g-tensor of the primary donor P700+. could be resolved and measured with high accuracy as g11 = 2.00304, g22 = 2.00262, and g33 = 2.00232. Other signals arising from Mn2+ and a dark signal from PS-II at g approximately 2.00266 are distinguished from the P700+. g-tensor powder pattern. The measured g values are compared with those of several bacterial reaction center donors.[1]

References

  1. Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles. Prisner, T.F., McDermott, A.E., Un, S., Norris, J.R., Thurnauer, M.C., Griffin, R.G. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
 
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