The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

About

Terms

 

 
 
 
 

Structure of the asn-linked oligosaccharides of apolipophorin III from the insect Locusta migratoria. Carbohydrate-linked 2-aminoethylphosphonate as a constituent of a glycoprotein.

The primary structures of the N-linked carbohydrate chains of apolipophorin III from the insect Locusta migratoria have been determined. The glycoprotein was completely deglycosylated with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. Released oligosaccharides were separated from the remaining protein using gel-permeation chromatography on Bio-Gel P-100. Purification of the carbohydrate chains was achieved by a combination of FPLC anion-exchange chromatography on Mono-Q and amine adsorption HPLC on Lichrosorb-NH2. The structures of the carbohydrate chains were deduced with a combination of fast atom bombardment mass spectrometry, 1H- and 31P-NMR spectroscopy, and methylation analysis. The majority of the carbohydrate chains contains 2-aminoethylphosphonate (AEP), which is linked to the 6-position of Man and/or GlcNAc. L. migratoria apolipophorin III is the first example of a glycoprotein containing carbohydrate-linked 2-aminoethylphosphonate. The structures of the major oligosaccharides were established to be the following: [formula: see text][1]

References

  1. Structure of the asn-linked oligosaccharides of apolipophorin III from the insect Locusta migratoria. Carbohydrate-linked 2-aminoethylphosphonate as a constituent of a glycoprotein. Hård, K., Van Doorn, J.M., Thomas-Oates, J.E., Kamerling, J.P., Van der Horst, D.J. Biochemistry (1993) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities