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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Flow-injection extraction-spectrophotometric determination of bromhexine with orange IV.

An automatic flow-injection photometric method for the determination of bromhexine is proposed. The drug was determined by formation of an ion-pair with orange IV, extraction into 1,2-dichloroethane and measurement of the absorbance at 412 nm of the organic phase. A linear calibration graph was obtained at concentrations of 5 x 10(-6)-1.6 x 10(-4) M of bromhexine. Up to 40 samples h-1 can be processed with an RSD of 0.32-0.88%. The method was applied to the determination of bromhexine in blood serum and a pharmaceutical preparation.[1]


  1. Flow-injection extraction-spectrophotometric determination of bromhexine with orange IV. Pérez-Ruiz, T., Martínez-Lozano, C., Sanz, A., Mondéjar, S. Journal of pharmaceutical and biomedical analysis. (1995) [Pubmed]
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