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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Characterization of a rice sucrose-phosphate synthase-encoding gene.

A rice genomic clone ( sps1) coding for sucrose phosphate synthase ( SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.[1]

References

  1. Characterization of a rice sucrose-phosphate synthase-encoding gene. Valdez-Alarcón, J.J., Ferrando, M., Salerno, G., Jimenez-Moraila, B., Herrera-Estrella, L. Gene (1996) [Pubmed]
 
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