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Lea, I.A. et al.

Lea, Richardson, Widgren, O'Rand,

Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.

In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated HSp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homology with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the protein to be 24.5 kDa.[1]

References

Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17. Lea, I.A., Richardson, R.T., Widgren, E.E., O'Rand, M.G. Biochim. Biophys. Acta (1996) [Pubmed]

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