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MeSH Review

Electrophoresis, Polyacrylamide Gel

 
 
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Disease relevance of Electrophoresis, Polyacrylamide Gel

 

Psychiatry related information on Electrophoresis, Polyacrylamide Gel

  • Recombinant tau phosphorylated by GSK-3 aligned on SDS-PAGE with the abnormally phosphorylated tau (PHF-tau) associated with the paired helical filaments in Alzheimer's disease brain [6].
 

High impact information on Electrophoresis, Polyacrylamide Gel

  • On SDS-PAGE, MCP migrates as two broad forms with Mrs of 59,000-68,000 and 51,000-58,000 [7].
  • Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphorylated protein that migrates at 62 kDa by SDS-PAGE and associates with the p120 ras GTPase-activating protein (GAP) [8].
  • The cross-linked cells were solubilized in Nonidet-P40, immunoprecipitated with anti-Ti (monoclonal antibody T40/25) or anti-T3 (monoclonal antibodies UCHT-1 or OKT3), and analyzed by SDS-PAGE [9].
  • The two-dimensional SDS-PAGE analysis (nonreducing followed by reducing conditions) of the DSS and BSOCOES cross-linked products revealed the specifically cross-linked bands to have Mr 40,000 and Mr 28,000 [9].
  • To initiate a genetic study of the hormonal regulation of genes coding for the three vitellogenins or yolk polypeptide precursors (YPs) in Drosophila, we have isolated from wild flies genetic variants which alter the mobility in SDS-PAGE of each YP independently [10].
 

Chemical compound and disease context of Electrophoresis, Polyacrylamide Gel

 

Biological context of Electrophoresis, Polyacrylamide Gel

 

Anatomical context of Electrophoresis, Polyacrylamide Gel

 

Associations of Electrophoresis, Polyacrylamide Gel with chemical compounds

  • Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions are consistent with the Ly-3.1 antigen containing a glycoprotein subunit with an apparent mol wt of 35,000 daltons [26].
  • Proteins in extracts of normal human epidermis were separated by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE), then transferred to nitrocellulose or 2-aminophenylthioether paper for immunoperoxidase staining [27].
  • The pattern obtained by subjecting the LPS to SDS-PAGE and staining the gel with silver nitrate suggests that L. pneumophila LPS might be atypical [28].
  • Some of the deposited C3 were complexed with membrane proteins, since 140- and 250-kD bands became significantly accumulated on SDS-PAGE by treatment with the antibodies [29].
  • In accordance with the hypothesis that C4b-associated C4b might also serve as a high-affinity C5-binding site, a small amount of C4b-C4b dimer was detected on EAC14 cells by SDS-PAGE analysis [30].
 

Gene context of Electrophoresis, Polyacrylamide Gel

  • The purified MCAF showed microheterogeneity yielding two bands on SDS-PAGE analysis [31].
  • It was found that the CD59 antigen is a small protein (approximately 20 kD as judged by SDS-PAGE, 11.5 kD predicted from the isolated cDNA) sometimes associated with larger components (45 and 80 kD) in urine [32].
  • Immunoblot analysis using a polyclonal antiserum against the cytoplasmic domain showed that beta pat-3 migrates in SDS-PAGE with apparent M(r) of 109 k and 120 k under nonreducing and reducing conditions, respectively [33].
  • Anillin migrates at 190 kD on SDS-PAGE [34].
  • NOP3-specific antibodies recognize a 60-kD protein by SDS-PAGE and decorate the nucleolus and the surrounding nucleoplasm [35].
 

Analytical, diagnostic and therapeutic context of Electrophoresis, Polyacrylamide Gel

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