A self-fertile mutant of Phalaris produces an S protein with reduced thioredoxin activity.
Gametophytic self-incompatibility in the Phalaris coerulescens is controlled by two unlinked genes, S and Z. Isolation of the S gene from the pollen of this grass species indicated that the C terminus has significant homology with thioredoxin H proteins. The protein from the C terminus, expressed in Escherichia coli, exhibits thioredoxin-life activity. This paper demonstrates that the C terminus of the S protein from an S complete mutant shows significant reduction in thioredoxin activity when compared with the wild-type form. Both pollen and stigma have lost self-compatibility in this mutant. Close examination of the lesions, which were found only in the C terminus of the mutant gene suggests that the substitution of a serine by an arginine is responsible for the reduced enzymatic activity. The association between reduced activity and the loss of the self-incompatibility provides evidence for a role of thioredoxin activity in the self-incompatibility reaction of this species.[1]References
- A self-fertile mutant of Phalaris produces an S protein with reduced thioredoxin activity. Li, X., Nield, J., Hayman, D., Langridge, P. Plant J. (1996) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg