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Cloning and expression of the chicken protein tyrosine phosphatase SH-PTP2.

SH-PTP2 is a protein tyrosine phosphatase which contains two src homology 2 (SH2) domains. A partial cDNA clone encoding chicken SH-PTP2 was generated by RT-PCR and used as a probe to screen several chicken cDNA libraries. Two overlapping cDNA clones were identified and the nucleotide sequence of chicken SH-PTP2 containing the entire protein-coding region was determined. The deduced amino acid sequence shares 98% and 94% identity, respectively with the corresponding human and Xenopus proteins. Northern and Western blot analyses show that chicken SH-PTP2 is expressed ubiquitously like those of mammals and Xenopus. This suggests that chicken SH-PTP2 may have analogous biological roles to those of mammals.[1]

References

  1. Cloning and expression of the chicken protein tyrosine phosphatase SH-PTP2. Park, C.Y., LaMontagne, K.R., Tonks, N.K., Hayman, M.J. Gene (1996) [Pubmed]
 
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