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MeSH Review

Reverse Transcriptase Polymerase Chain Reaction

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Disease relevance of Reverse Transcriptase Polymerase Chain Reaction


Psychiatry related information on Reverse Transcriptase Polymerase Chain Reaction


High impact information on Reverse Transcriptase Polymerase Chain Reaction

  • Single-cell RT-PCR documented that individual cells express several different Dscam isoforms and allowed an estimation of the diversity that is present [11].
  • Bone marrow mononuclear cells were serially monitored by flow cytometry for immunophenotype, fluorescence in situ hybridization, reverse-transcription-polymerase-chain-reaction (RT-PCR) assay for PML-RAR-alpha fusion transcripts, and Western blot analysis for expression of the apoptosis-associated proteins caspases 1, 2, and 3 [12].
  • Using RT-PCR, northern and sequence analyses, we now demonstrate that X25 comprises part of the STM7 gene, contributing to at least four splice variants, and report the identification of new coding sequences [13].
  • Furthermore, RT-PCR analysis reveals internally deleted and mutated forms of the NAIP transcript in type I SMA individuals and not in unaffected individuals [14].
  • Cloning and sequencing of the RT-PCR product showed fusion points between EWS and FLI1 cDNA identical to those detected in Ewing tumors [15].

Chemical compound and disease context of Reverse Transcriptase Polymerase Chain Reaction


Biological context of Reverse Transcriptase Polymerase Chain Reaction


Anatomical context of Reverse Transcriptase Polymerase Chain Reaction

  • PU.1 is completely absent from peripheral T cells and most T cell lines based on sensitive RT-PCR assays [26].
  • Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we have demonstrated in the present study that IL-15 and its cognate receptor, but not the other cytokines, are expressed in the mouse pregnant uterus, with a time course concomitant with those of cytolytic mediators in differentiated GMG cells [27].
  • METHODS: We assessed GRPR messenger RNA (mRNA) expression in specimens from 25 patients with SCCHN, six control noncancer patients, and 14 SCCHN cell lines by use of quantitative reverse transcriptase-polymerase chain reaction [28].
  • In this study, by using a binding assay and a competitive reverse-transcriptase polymerase chain reaction, we examined the regulation of AngII types 1a and 1b (AT1a-R and AT1b-R) and type 2 receptor (AT2-R) expression in embryonal day 19 (E19) and neonatal (1-d) rat cardiac fibroblasts and cardiomyocytes [29].
  • Using RT-PCR, TNF was detected in human heart, in skeletal muscle from humans and rats, and in cultured human myocytes [30].

Associations of Reverse Transcriptase Polymerase Chain Reaction with chemical compounds


Gene context of Reverse Transcriptase Polymerase Chain Reaction


Analytical, diagnostic and therapeutic context of Reverse Transcriptase Polymerase Chain Reaction


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