The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Reverse Transcriptase Polymerase Chain Reaction

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of Reverse Transcriptase Polymerase Chain Reaction

 

Psychiatry related information on Reverse Transcriptase Polymerase Chain Reaction

 

High impact information on Reverse Transcriptase Polymerase Chain Reaction

  • Single-cell RT-PCR documented that individual cells express several different Dscam isoforms and allowed an estimation of the diversity that is present [11].
  • Bone marrow mononuclear cells were serially monitored by flow cytometry for immunophenotype, fluorescence in situ hybridization, reverse-transcription-polymerase-chain-reaction (RT-PCR) assay for PML-RAR-alpha fusion transcripts, and Western blot analysis for expression of the apoptosis-associated proteins caspases 1, 2, and 3 [12].
  • Using RT-PCR, northern and sequence analyses, we now demonstrate that X25 comprises part of the STM7 gene, contributing to at least four splice variants, and report the identification of new coding sequences [13].
  • Furthermore, RT-PCR analysis reveals internally deleted and mutated forms of the NAIP transcript in type I SMA individuals and not in unaffected individuals [14].
  • Cloning and sequencing of the RT-PCR product showed fusion points between EWS and FLI1 cDNA identical to those detected in Ewing tumors [15].
 

Chemical compound and disease context of Reverse Transcriptase Polymerase Chain Reaction

 

Biological context of Reverse Transcriptase Polymerase Chain Reaction

 

Anatomical context of Reverse Transcriptase Polymerase Chain Reaction

  • PU.1 is completely absent from peripheral T cells and most T cell lines based on sensitive RT-PCR assays [26].
  • Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we have demonstrated in the present study that IL-15 and its cognate receptor, but not the other cytokines, are expressed in the mouse pregnant uterus, with a time course concomitant with those of cytolytic mediators in differentiated GMG cells [27].
  • METHODS: We assessed GRPR messenger RNA (mRNA) expression in specimens from 25 patients with SCCHN, six control noncancer patients, and 14 SCCHN cell lines by use of quantitative reverse transcriptase-polymerase chain reaction [28].
  • In this study, by using a binding assay and a competitive reverse-transcriptase polymerase chain reaction, we examined the regulation of AngII types 1a and 1b (AT1a-R and AT1b-R) and type 2 receptor (AT2-R) expression in embryonal day 19 (E19) and neonatal (1-d) rat cardiac fibroblasts and cardiomyocytes [29].
  • Using RT-PCR, TNF was detected in human heart, in skeletal muscle from humans and rats, and in cultured human myocytes [30].
 

Associations of Reverse Transcriptase Polymerase Chain Reaction with chemical compounds

 

Gene context of Reverse Transcriptase Polymerase Chain Reaction

 

Analytical, diagnostic and therapeutic context of Reverse Transcriptase Polymerase Chain Reaction

References

  1. Micrometastases and survival in stage II colorectal cancer. Liefers, G.J., Cleton-Jansen, A.M., van de Velde, C.J., Hermans, J., van Krieken, J.H., Cornelisse, C.J., Tollenaar, R.A. N. Engl. J. Med. (1998) [Pubmed]
  2. SYT-SSX gene fusion as a determinant of morphology and prognosis in synovial sarcoma. Kawai, A., Woodruff, J., Healey, J.H., Brennan, M.F., Antonescu, C.R., Ladanyi, M. N. Engl. J. Med. (1998) [Pubmed]
  3. Molecular staging of malignant melanoma: correlation with clinical outcome. Shivers, S.C., Wang, X., Li, W., Joseph, E., Messina, J., Glass, L.F., DeConti, R., Cruse, C.W., Berman, C., Fenske, N.A., Lyman, G.H., Reintgen, D.S. JAMA (1998) [Pubmed]
  4. Residual HIV-1 RNA in blood plasma of patients taking suppressive highly active antiretroviral therapy. Dornadula, G., Zhang, H., VanUitert, B., Stern, J., Livornese, L., Ingerman, M.J., Witek, J., Kedanis, R.J., Natkin, J., DeSimone, J., Pomerantz, R.J. JAMA (1999) [Pubmed]
  5. A proinflammatory activity of interleukin 8 in human skin: expression of the inducible nitric oxide synthase in psoriatic lesions and cultured keratinocytes. Bruch-Gerharz, D., Fehsel, K., Suschek, C., Michel, G., Ruzicka, T., Kolb-Bachofen, V. J. Exp. Med. (1996) [Pubmed]
  6. Elevated central nervous system prostaglandins in human immunodeficiency virus-associated dementia. Griffin, D.E., Wesselingh, S.L., McArthur, J.C. Ann. Neurol. (1994) [Pubmed]
  7. Up-regulated production and activation of the complement system in Alzheimer's disease brain. Yasojima, K., Schwab, C., McGeer, E.G., McGeer, P.L. Am. J. Pathol. (1999) [Pubmed]
  8. Gonadotropin-releasing hormone receptor gene expression in human ovary and granulosa-lutein cells. Minaretzis, D., Jakubowski, M., Mortola, J.F., Pavlou, S.N. J. Clin. Endocrinol. Metab. (1995) [Pubmed]
  9. Role of connective tissue growth factor in mediating hypertrophy of human proximal tubular cells induced by angiotensin II. Liu, B.C., Sun, J., Chen, Q., Ma, K.L., Ruan, X.Z., Phillips, A.O. American journal of nephrology. (2003) [Pubmed]
  10. Cyclooxygenase-1 and -2 in brains of patients who died with sporadic Creutzfeldt-Jakob disease. Deininger, M.H., Bekure-Nemariam, K., Trautmann, K., Morgalla, M., Meyermann, R., Schluesener, H.J. J. Mol. Neurosci. (2003) [Pubmed]
  11. Stochastic yet biased expression of multiple Dscam splice variants by individual cells. Neves, G., Zucker, J., Daly, M., Chess, A. Nat. Genet. (2004) [Pubmed]
  12. Complete remission after treatment of acute promyelocytic leukemia with arsenic trioxide. Soignet, S.L., Maslak, P., Wang, Z.G., Jhanwar, S., Calleja, E., Dardashti, L.J., Corso, D., DeBlasio, A., Gabrilove, J., Scheinberg, D.A., Pandolfi, P.P., Warrell, R.P. N. Engl. J. Med. (1998) [Pubmed]
  13. The Friedreich's ataxia gene encodes a novel phosphatidylinositol-4- phosphate 5-kinase. Carvajal, J.J., Pook, M.A., dos Santos, M., Doudney, K., Hillermann, R., Minogue, S., Williamson, R., Hsuan, J.J., Chamberlain, S. Nat. Genet. (1996) [Pubmed]
  14. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Roy, N., Mahadevan, M.S., McLean, M., Shutler, G., Yaraghi, Z., Farahani, R., Baird, S., Besner-Johnston, A., Lefebvre, C., Kang, X. Cell (1995) [Pubmed]
  15. An association between viral genes and human oncogenic alterations: the adenovirus E1A induces the Ewing tumor fusion transcript EWS-FLI1. Sanchez-Prieto, R., de Alava, E., Palomino, T., Guinea, J., Fernandez, V., Cebrian, S., LLeonart, M., Cabello, P., Martin, P., San Roman, C., Bornstein, R., Pardo, J., Martinez, A., Diaz-Espada, F., Barrios, Y., Ramon y Cajal, S. Nat. Med. (1999) [Pubmed]
  16. Growth regulation of human prostate cancer cells by bone morphogenetic protein-2. Ide, H., Yoshida, T., Matsumoto, N., Aoki, K., Osada, Y., Sugimura, T., Terada, M. Cancer Res. (1997) [Pubmed]
  17. Altered gene expression pattern in cultured human breast cancer cells treated with hepatocyte growth factor/scatter factor in the setting of DNA damage. Yuan, R., Fan, S., Achary, M., Stewart, D.M., Goldberg, I.D., Rosen, E.M. Cancer Res. (2001) [Pubmed]
  18. API2-MALT1 fusion transcripts involved in mucosa-associated lymphoid tissue lymphoma: multiplex RT-PCR detection using formalin-fixed paraffin-embedded specimens. Inagaki, H., Okabe, M., Seto, M., Nakamura, S., Ueda, R., Eimoto, T. Am. J. Pathol. (2001) [Pubmed]
  19. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans. Berndt, J., Klöting, N., Kralisch, S., Kovacs, P., Fasshauer, M., Schön, M.R., Stumvoll, M., Blüher, M. Diabetes (2005) [Pubmed]
  20. Comparative studies of the estrogen receptors beta and alpha and the androgen receptor in normal human prostate glands, dysplasia, and in primary and metastatic carcinoma. Leav, I., Lau, K.M., Adams, J.Y., McNeal, J.E., Taplin, M.E., Wang, J., Singh, H., Ho, S.M. Am. J. Pathol. (2001) [Pubmed]
  21. Muscarinic and beta-adrenergic regulation of heart rate, force of contraction and calcium current is preserved in mice lacking endothelial nitric oxide synthase. Vandecasteele, G., Eschenhagen, T., Scholz, H., Stein, B., Verde, I., Fischmeister, R. Nat. Med. (1999) [Pubmed]
  22. The Fas counterattack: Fas-mediated T cell killing by colon cancer cells expressing Fas ligand. O'Connell, J., O'Sullivan, G.C., Collins, J.K., Shanahan, F. J. Exp. Med. (1996) [Pubmed]
  23. Cytokine transcriptional events during helper T cell subset differentiation. Lederer, J.A., Perez, V.L., DesRoches, L., Kim, S.M., Abbas, A.K., Lichtman, A.H. J. Exp. Med. (1996) [Pubmed]
  24. A defect in nucleosome remodeling prevents IL-12(p35) gene transcription in neonatal dendritic cells. Goriely, S., Van Lint, C., Dadkhah, R., Libin, M., De Wit, D., Demonté, D., Willems, F., Goldman, M. J. Exp. Med. (2004) [Pubmed]
  25. Localization of distinct Peyer's patch dendritic cell subsets and their recruitment by chemokines macrophage inflammatory protein (MIP)-3alpha, MIP-3beta, and secondary lymphoid organ chemokine. Iwasaki, A., Kelsall, B.L. J. Exp. Med. (2000) [Pubmed]
  26. The Ets protein Spi-B is expressed exclusively in B cells and T cells during development. Su, G.H., Ip, H.S., Cobb, B.S., Lu, M.M., Chen, H.M., Simon, M.C. J. Exp. Med. (1996) [Pubmed]
  27. The involvement of interleukin (IL)-15 in regulating the differentiation of granulated metrial gland cells in mouse pregnant uterus. Ye, W., Zheng, L.M., Young, J.D., Liu, C.C. J. Exp. Med. (1996) [Pubmed]
  28. Gastrin-releasing peptide receptor-mediated autocrine growth in squamous cell carcinoma of the head and neck. Lango, M.N., Dyer, K.F., Lui, V.W., Gooding, W.E., Gubish, C., Siegfried, J.M., Grandis, J.R. J. Natl. Cancer Inst. (2002) [Pubmed]
  29. Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture. Matsubara, H., Kanasaki, M., Murasawa, S., Tsukaguchi, Y., Nio, Y., Inada, M. J. Clin. Invest. (1994) [Pubmed]
  30. The expression of TNF alpha by human muscle. Relationship to insulin resistance. Saghizadeh, M., Ong, J.M., Garvey, W.T., Henry, R.R., Kern, P.A. J. Clin. Invest. (1996) [Pubmed]
  31. Effect of the laccase gene CNLAC1, on virulence of Cryptococcus neoformans. Salas, S.D., Bennett, J.E., Kwon-Chung, K.J., Perfect, J.R., Williamson, P.R. J. Exp. Med. (1996) [Pubmed]
  32. Regulation of angiotensin II receptor AT1 subtypes in renal afferent arterioles during chronic changes in sodium diet. Ruan, X., Wagner, C., Chatziantoniou, C., Kurtz, A., Arendshorst, W.J. J. Clin. Invest. (1997) [Pubmed]
  33. IL-1 produced and released endogenously within human islets inhibits beta cell function. Arnush, M., Heitmeier, M.R., Scarim, A.L., Marino, M.H., Manning, P.T., Corbett, J.A. J. Clin. Invest. (1998) [Pubmed]
  34. Oxysterols present in atherosclerotic tissue decrease the expression of lipoprotein lipase messenger RNA in human monocyte-derived macrophages. Hultén, L.M., Lindmark, H., Diczfalusy, U., Björkhem, I., Ottosson, M., Liu, Y., Bondjers, G., Wiklund, O. J. Clin. Invest. (1996) [Pubmed]
  35. alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer. Rubin, M.A., Zhou, M., Dhanasekaran, S.M., Varambally, S., Barrette, T.R., Sanda, M.G., Pienta, K.J., Ghosh, D., Chinnaiyan, A.M. JAMA (2002) [Pubmed]
  36. Shear-dependent eosinophil transmigration on interleukin 4-stimulated endothelial cells: a role for endothelium-associated eotaxin-3. Cuvelier, S.L., Patel, K.D. J. Exp. Med. (2001) [Pubmed]
  37. IL-4-deficient Balb/c mice resist infection with Leishmania major. Kopf, M., Brombacher, F., Köhler, G., Kienzle, G., Widmann, K.H., Lefrang, K., Humborg, C., Ledermann, B., Solbach, W. J. Exp. Med. (1996) [Pubmed]
  38. Simultaneous expression of tissue factor and tissue factor pathway inhibitor by human monocytes. A potential mechanism for localized control of blood coagulation. McGee, M.P., Foster, S., Wang, X. J. Exp. Med. (1994) [Pubmed]
  39. Expression of vascular endothelial growth factor and its receptors flt and KDR in ovarian carcinoma. Boocock, C.A., Charnock-Jones, D.S., Sharkey, A.M., McLaren, J., Barker, P.J., Wright, K.A., Twentyman, P.R., Smith, S.K. J. Natl. Cancer Inst. (1995) [Pubmed]
  40. Novel perspectives on pituitary and brain angiotensinogen. Sernia, C., Zeng, T., Kerr, D., Wyse, B. Frontiers in neuroendocrinology. (1997) [Pubmed]
  41. Glucocorticoid-suppressible hyperaldosteronism and adrenal tumors occurring in a single French pedigree. Pascoe, L., Jeunemaitre, X., Lebrethon, M.C., Curnow, K.M., Gomez-Sanchez, C.E., Gasc, J.M., Saez, J.M., Corvol, P. J. Clin. Invest. (1995) [Pubmed]
  42. Transduction of the IL2 gene into human acute leukemia cells: induction of tumor rejection without modifying cell proliferation and IL2 receptor expression. Cignetti, A., Guarini, A., Carbone, A., Forni, M., Cronin, K., Forni, G., Gansbacher, B., Foa, R. J. Natl. Cancer Inst. (1994) [Pubmed]
  43. Retinoid signaling controls mouse pancreatic exocrine lineage selection through epithelial-mesenchymal interactions. Kobayashi, H., Spilde, T.L., Bhatia, A.M., Buckingham, R.B., Hembree, M.J., Prasadan, K., Preuett, B.L., Imamura, M., Gittes, G.K. Gastroenterology (2002) [Pubmed]
 
WikiGenes - Universities