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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody.

Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.[1]

References

  1. A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody. Kobatake, E., Sasakura, H., Haruyama, T., Laukkanen, M.L., Keinänen, K., Aizawa, M. Anal. Chem. (1997) [Pubmed]
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