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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Human endothelial culture supernatant selectively promotes IgM-producing hybridomas.

We compared the effect of human endothelial cell culture supernatant (HECS), ESG (Ewing sarcoma growth factor), IL-6 (interleukin-6) and peritoneal macrophages on the recovery of hybridoma cells after fusion with respect to growth, stability and distribution of isotype variants. A selective growth of murine IgM-producing hybridoma cells was observed in the presence of HECS after cell fusion.[1]

References

  1. Human endothelial culture supernatant selectively promotes IgM-producing hybridomas. Greferath, R., Sauer-Nehls, S., Dyballa, S., Nehls, P. J. Immunol. Methods (1997) [Pubmed]
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