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Disease relevance of Hybridomas


Psychiatry related information on Hybridomas

  • The BALB/cV immune response to fluorescein (F1) was analyzed for expression of serologically defined idiotypes (Id) present on two anti-F1 hybridoma proteins, 3-13 and 3-17 [6].

High impact information on Hybridomas

  • Several other experiments indicated that IL-2 production in these hybridomas is triggered via TcR gamma delta [7].
  • Messenger RNA was prepared from a hybridoma cell line secreting a monoclonal antibody (53FC3) directed against a luminal epitope of a Golgi membrane protein (Mr = 135 kd) found in rodent cells [8].
  • The fine specificity of antigen and Ia determinant recognition by T cell hybridoma clones specific for pigeon cytochrome c [9].
  • Amino acid sequence analyses of heavy-chain variable (VH) regions from 19 myeloma and hybridoma immunoglobulins binding phosphorylcholine show that 10 are identical (the prototype T15 VH sequence) and 9 are distinct variants differing by one to eight residues [10].
  • An alternative mechanism for the expression of the delta gene is found in hybridoma B1-8. delta 1, which actively secretes delta chains and synthesizes no mu chain [11].

Chemical compound and disease context of Hybridomas


Biological context of Hybridomas


Anatomical context of Hybridomas

  • B-cell hybridoma lines show that a negative feedback inhibition of mu and kappa transgenic products on the immunoglobulin heavy-chain rearrangement is possible [21].
  • Pre-B lymphocytes, and hybridomas derived from them, synthesize immunoglobulin heavy (IgH) chain in the absence of light (L) chain [22].
  • The antigen-specific response of T hybridoma cells expressing human CD2trans- protein was enhanced up to 400% when the human LFA-3 ligand was introduced into the I-Ad expressing murine antigen-presenting cells [23].
  • LFA-1 but not Lyt-2 is associated with killing activity of cytotoxic T lymphocyte hybridomas [24].
  • Analysis of the components of one such rearranged gene, 2B4, isolated from the pigeon cytochrome c-specific, H-2E-restricted T helper (TH) hybridoma, and its unrearranged (liver) counterpart, indicate that an 8-nucleotide sequence 3' to the rearranged variable region is not derived from either the germ-line V- or J-region gene segments [25].

Associations of Hybridomas with chemical compounds

  • Here, we show that a fluorescein-labelled synthetic peptide, together with Texas red-labelled class II MHC antigen, I-Ad, stimulates the production of interleukin-2 by a peptide-specific I-Ad-restricted T-cell hybridoma when reconstituted in a lipid membrane on a glass substrate [26].
  • We compared the physical constants of hapten binding by antibodies from 2-phenyl-5-oxazolone-specific hybridomas from primary, secondary and tertiary responses [27].
  • A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsonate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI) [28].
  • Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). II. Comparison of hybridomas derived by lipopolysaccharide stimulation and secondary protein immunization [29].
  • In the parent hybridoma, cross-linking of the CD8 alpha chain led to minimal enhancement of CD8-associated Lck tyrosine kinase activity [30].

Gene context of Hybridomas

  • Because T-cell receptor ligation can induce apoptosis in a single T hybridoma cell, we suggest that the Fas/Fas-ligand interaction can induce cell death in a cell-autonomous manner [31].
  • A similar relationship in the patterns of lymphokines produced by activated T cell hybridomas maintained under serum-free conditions was also observed, whereas activation in serum-supplemented media resulted in a predominant restriction to the secretion of IL-2 [32].
  • T cell growth and differentiation induced by interleukin-HP1/IL-6, the murine hybridoma/plasmacytoma growth factor [33].
  • Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma [34].
  • Consistent with these results, IL-2 production by the mCD1.1-specific T cell hybridoma DN32.D3 was induced by thymocytes from normal mice or mice with a homozygous deletion of the TAP1 gene, but not by thymocytes from mice with a homozygous deletion of the beta 2m gene [35].

Analytical, diagnostic and therapeutic context of Hybridomas


  1. CH gene rearrangements in IgM-bearing B cells and in the normal splenic DNA component of hybridomas making different isotypes of antibody. Hurwitz, J.L., Coleclough, C., Cebra, J.J. Cell (1980) [Pubmed]
  2. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Linzer, D.I., Levine, A.J. Cell (1979) [Pubmed]
  3. H-2K-, H-21- and H-2D-restricted hybridoma contact sensitivity effector cells. Minami, M., Okuda, K., Sunday, M.E., Dorf, M.E. Nature (1982) [Pubmed]
  4. Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. Shimonkevitz, R., Kappler, J., Marrack, P., Grey, H. J. Exp. Med. (1983) [Pubmed]
  5. The single positive T cells found in CD3-zeta/eta-/- mice overtly react with self-major histocompatibility complex molecules upon restoration of normal surface density of T cell receptor-CD3 complex. Lin, S.Y., Ardouin, L., Gillet, A., Malissen, M., Malissen, B. J. Exp. Med. (1997) [Pubmed]
  6. Identification of recurrent idiotypes within the unrestricted anti-fluorescein immune response. Reinitz, D.M., Voss, E.W. J. Immunol. (1985) [Pubmed]
  7. Stimulation of a major subset of lymphocytes expressing T cell receptor gamma delta by an antigen derived from Mycobacterium tuberculosis. O'Brien, R.L., Happ, M.P., Dallas, A., Palmer, E., Kubo, R., Born, W.K. Cell (1989) [Pubmed]
  8. Microinjection of mRNA coding for an anti-Golgi antibody inhibits intracellular transport of a viral membrane protein. Burke, B., Warren, G. Cell (1984) [Pubmed]
  9. The fine specificity of antigen and Ia determinant recognition by T cell hybridoma clones specific for pigeon cytochrome c. Hedrick, S.M., Matis, L.A., Hecht, T.T., Samelson, L.E., Longo, D.L., Heber-Katz, E., Schwartz, R.H. Cell (1982) [Pubmed]
  10. Antibody diversity: somatic hypermutation of rearranged VH genes. Kim, S., Davis, M., Sinn, E., Patten, P., Hood, L. Cell (1981) [Pubmed]
  11. The role of DNA rearrangement and alternative RNA processing in the expression of immunoglobulin delta genes. Maki, R., Roeder, W., Traunecker, A., Sidman, C., Wabl, M., Raschke, W., Tonegawa, S. Cell (1981) [Pubmed]
  12. Class II major histocompatibility complex-restricted T cells specific for a virion structural protein that do not recognize exogenous influenza virus. Evidence that presentation of labile T cell determinants is favored by endogenous antigen synthesis. Eisenlohr, L.C., Hackett, C.J. J. Exp. Med. (1989) [Pubmed]
  13. Retinoic acid induces the differentiation of B cell hybridomas from patients with common variable immunodeficiency. Sherr, E., Adelman, D.C., Saxon, A., Gilly, M., Wall, R., Sidell, N. J. Exp. Med. (1988) [Pubmed]
  14. Antibacterial activity of a human monoclonal antibody to haemophilus influenzae type B capsular polysaccharide. Hunter, K.W., Fischer, G.W., Hemming, V.G., Wilson, S.R., Hartzman, R.J., Woody, J.N. Lancet (1982) [Pubmed]
  15. Antibody-mediated activation of genetically defective Escherichia coli beta-galactosidases by monoclonal antibodies produced by somatic cell hybrids. Accolla, R.S., Cina, R., Montesoro, E., Celada, F. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  16. Expression of pertussis toxin adenosine diphosphate-ribosyltransferase in a T-cell hybridoma reduces metastatic capacity. Driessens, M.H., van Rijthoven, E.A., La Rivière, G., Roos, E. Blood (1996) [Pubmed]
  17. Requirement for the orphan steroid receptor Nur77 in apoptosis of T-cell hybridomas. Woronicz, J.D., Calnan, B., Ngo, V., Winoto, A. Nature (1994) [Pubmed]
  18. Cyclosporin A inhibits activation-induced cell death in T-cell hybridomas and thymocytes. Shi, Y.F., Sahai, B.M., Green, D.R. Nature (1989) [Pubmed]
  19. Monoclonal antibodies identify a cell-surface antigen associated with an activated cellular oncogene. Drebin, J.A., Stern, D.F., Link, V.C., Weinberg, R.A., Greene, M.I. Nature (1984) [Pubmed]
  20. The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Brunet, J.F., Dosseto, M., Denizot, F., Mattei, M.G., Clark, W.R., Haqqi, T.M., Ferrier, P., Nabholz, M., Schmitt-Verhulst, A.M., Luciani, M.F. Nature (1986) [Pubmed]
  21. Transmission and expression of a specific pair of rearranged immunoglobulin mu and kappa genes in a transgenic mouse line. Rusconi, S., Köhler, G. Nature (1985) [Pubmed]
  22. Immunoglobulin heavy chain binding protein. Haas, I.G., Wabl, M. Nature (1983) [Pubmed]
  23. CD2-mediated adhesion facilitates T lymphocyte antigen recognition function. Moingeon, P., Chang, H.C., Wallner, B.P., Stebbins, C., Frey, A.Z., Reinherz, E.L. Nature (1989) [Pubmed]
  24. LFA-1 but not Lyt-2 is associated with killing activity of cytotoxic T lymphocyte hybridomas. Kaufmann, Y., Golstein, P., Pierres, M., Springer, T.A., Eshhar, Z. Nature (1982) [Pubmed]
  25. Localization of a T-cell receptor diversity-region element. Kavaler, J., Davis, M.M., Chien, Y. Nature (1984) [Pubmed]
  26. T-cell-mediated association of peptide antigen and major histocompatibility complex protein detected by energy transfer in an evanescent wave-field. Watts, T.H., Gaub, H.E., McConnell, H.M. Nature (1986) [Pubmed]
  27. Kinetic maturation of an immune response. Foote, J., Milstein, C. Nature (1991) [Pubmed]
  28. Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain. Sims, J., Rabbitts, T.H., Estess, P., Slaughter, C., Tucker, P.W., Capra, J.D. Science (1982) [Pubmed]
  29. Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). II. Comparison of hybridomas derived by lipopolysaccharide stimulation and secondary protein immunization. Shlomchik, M., Nemazee, D., van Snick, J., Weigert, M. J. Exp. Med. (1987) [Pubmed]
  30. CD8 beta chain influences CD8 alpha chain-associated Lck kinase activity. Irie, H.Y., Ravichandran, K.S., Burakoff, S.J. J. Exp. Med. (1995) [Pubmed]
  31. Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas. Brunner, T., Mogil, R.J., LaFace, D., Yoo, N.J., Mahboubi, A., Echeverri, F., Martin, S.J., Force, W.R., Lynch, D.H., Ware, C.F. Nature (1995) [Pubmed]
  32. Platelet-derived growth factor is a potent biologic response modifier of T cells. Daynes, R.A., Dowell, T., Araneo, B.A. J. Exp. Med. (1991) [Pubmed]
  33. T cell growth and differentiation induced by interleukin-HP1/IL-6, the murine hybridoma/plasmacytoma growth factor. Uyttenhove, C., Coulie, P.G., Van Snick, J. J. Exp. Med. (1988) [Pubmed]
  34. Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma. Vandenabeele, P., Declercq, W., Vercammen, D., Van de Craen, M., Grooten, J., Loetscher, H., Brockhaus, M., Lesslauer, W., Fiers, W. J. Exp. Med. (1992) [Pubmed]
  35. TAP-independent, beta 2-microglobulin-dependent surface expression of functional mouse CD1.1. Brutkiewicz, R.R., Bennink, J.R., Yewdell, J.W., Bendelac, A. J. Exp. Med. (1995) [Pubmed]
  36. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Gearhart, P.J., Johnson, N.D., Douglas, R., Hood, L. Nature (1981) [Pubmed]
  37. The SJL/J T cell response to both spontaneous and transplantable syngeneic reticulum cell sarcoma is mediated predominantly by the V beta 17a+ T cell clonotype. Katz, J.D., Ohnishi, K., Lebow, L.T., Bonavida, B. J. Exp. Med. (1988) [Pubmed]
  38. Development of a human T-T cell hybridoma secreting B cell growth factor. Butler, J.L., Muraguchi, A., Lane, H.C., Fauci, A.S. J. Exp. Med. (1983) [Pubmed]
  39. Genetic basis for expression of the idiotypic network. One unique Ig VH germline gene accounts for the major family of Ab1 and Ab3 (Ab1') antibodies of the GAT system. Schiff, C., Milili, M., Hue, I., Rudikoff, S., Fougereau, M. J. Exp. Med. (1986) [Pubmed]
  40. Specific binding of fibronectin--antifibronectin immune complexes to procollagen: a new pitfall in immunostaining. McDonald, J.A., Kelley, D.G. J. Cell Biol. (1984) [Pubmed]
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