One-step HPLC purification procedure for porcine brain 90-kDa heat shock protein.
The 90-kDa heat shock protein (HSP90) was purified from porcine brain by a novel single-step purification procedure using diethylaminoethyl high-performance liquid chromatography (HPLC). About 4.8 mg of HSP90 was isolated from 25 g wet wt porcine brain tissue. The purified protein possessed a single moiety on one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining. Western blotting using monoclonal antibody prepared against human HSP90 confirmed its identity as HSP90. These results indicate that small-scale HPLC purification of HSP90 from porcine brain tissue can be readily accomplished, with high yield, using a convenient one-step purification method. The procedure described in this paper represents a significant improvement in current purification methods for the isolation of HSP90 from porcine brain.[1]References
- One-step HPLC purification procedure for porcine brain 90-kDa heat shock protein. Chu, L.F., Lee, W.C., Yang, P.C., Chu, R., Huang, T.Y., Mao, S.J. Protein Expr. Purif. (1997) [Pubmed]
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