The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Silver Staining

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of Silver Staining

 

Psychiatry related information on Silver Staining

 

High impact information on Silver Staining

  • Histological analysis of micrometastases in the lung on day 5 by silver staining of proteins associated with nucleolar organizer regions revealed more metastatic foci and augmented proliferative activity of the tumor cells after rmTNF pretreatment of mice [8].
  • The 150-kD glycoprotein was the only protein from 32D cl 3 cells, which was detectable by silver-staining after a one-step affinity-isolation [9].
  • Quantification by metabolic radiolabeling with [35S]methionine or by silver staining revealed a 3.0-3.5-fold increase in MAP1.2 levels relative to total cell protein after NGF treatment for 2 wk or longer [10].
  • The major SAF protein (PrP33-35) and its normal brain homologue are shown to have the same apparent mol. wt and ionic charge distribution by two-dimensional gel analysis, silver staining and immunoblotting [11].
  • Purpurin and the serum RBP are, however, different molecules, for the serum protein is approximately 3,000 D larger than purpurin and has different silver-staining characteristics [12].
 

Chemical compound and disease context of Silver Staining

 

Biological context of Silver Staining

 

Anatomical context of Silver Staining

 

Associations of Silver Staining with chemical compounds

  • Our modified procedure for staining total protein on nitrocellulose is rapid, inexpensive, and as sensitive as silver-staining of polyacrylamide gels [27].
  • The combination of nonequilibrium isoelectric focusing (IEF) in the first dimension, SDS electrophoresis in the second dimension, and very sensitive silver staining techniques resolves more than 250 individual protein spots [28].
  • Selective and sensitive silver staining of extracellular amyloid deposits and intraneuronal neurofibrillary changes can be applied to 50-150 microns thick polyethylene glycol sections and/or 5-15 microns thick paraffin sections [29].
  • During the past two years, the performance of fluorescence-based protein detection methods has demonstrably eclipsed conventional technologies such as colloidal Coomassie Blue and silver staining with respect to detection sensitivity, quantitative accuracy and compatibility with modern protein identification and characterization procedures [30].
  • After being stained with Coomassie Brilliant Blue or a silver-staining solution, each purified enzyme showed a single protein band on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate [31].
 

Gene context of Silver Staining

 

Analytical, diagnostic and therapeutic context of Silver Staining

References

  1. Accumulation of beta-amyloid precursor protein in HIV encephalitis: relationship with neuropsychological abnormalities. Giometto, B., An, S.F., Groves, M., Scaravilli, T., Geddes, J.F., Miller, R., Tavolato, B., Beckett, A.A., Scaravilli, F. Ann. Neurol. (1997) [Pubmed]
  2. Persistent grossly elevated plasma immunoglobulin A levels in untreated streptozocin-induced diabetic rats. Miller, L.L., Izzo, M.J., Wemett, D. Diabetes (1988) [Pubmed]
  3. Indium 111-monoclonal antimyosin antibody and magnetic resonance imaging in the diagnosis of acute Lyme myopericarditis. Bergler-Klein, J., Sochor, H., Stanek, G., Globits, S., Ullrich, R., Glogar, D. Arch. Intern. Med. (1993) [Pubmed]
  4. Further characterization of a protein kinase from foot-and-mouth disease virus. Grubman, M.J. J. Virol. (1982) [Pubmed]
  5. Nucleolin is an Ag-NOR protein; this property is determined by its amino-terminal domain independently of its phosphorylation state. Roussel, P., Belenguer, P., Amalric, F., Hernandez-Verdun, D. Exp. Cell Res. (1992) [Pubmed]
  6. Amyloid beta protein deposition in patients with frontotemporal lobar degeneration: relationship to age and apolipoprotein E genotype. Mann, D.M., McDonagh, A.M., Pickering-Brown, S.M., Kowa, H., Iwatsubo, T. Neurosci. Lett. (2001) [Pubmed]
  7. Comparative analysis of an improved thioflavin-s stain, Gallyas silver stain, and immunohistochemistry for neurofibrillary tangle demonstration on the same sections. Sun, A., Nguyen, X.V., Bing, G. J. Histochem. Cytochem. (2002) [Pubmed]
  8. Enhancement of experimental metastasis by tumor necrosis factor. Orosz, P., Echtenacher, B., Falk, W., Rüschoff, J., Weber, D., Männel, D.N. J. Exp. Med. (1993) [Pubmed]
  9. Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells. Levinovitz, A., Mühlhoff, J., Isenmann, S., Vestweber, D. J. Cell Biol. (1993) [Pubmed]
  10. Nerve growth factor regulates both the phosphorylation and steady-state levels of microtubule-associated protein 1.2 (MAP1.2). Aletta, J.M., Lewis, S.A., Cowan, N.J., Greene, L.A. J. Cell Biol. (1988) [Pubmed]
  11. The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal protein sequence as predicted for the normal brain protein (PrP). Hope, J., Morton, L.J., Farquhar, C.F., Multhaup, G., Beyreuther, K., Kimberlin, R.H. EMBO J. (1986) [Pubmed]
  12. A chick neural retina adhesion and survival molecule is a retinol-binding protein. Schubert, D., LaCorbiere, M., Esch, F. J. Cell Biol. (1986) [Pubmed]
  13. Electrophoretic heterogeneity and interstrain variation of the lipopolysaccharide of Haemophilus influenzae. Inzana, T.J. J. Infect. Dis. (1983) [Pubmed]
  14. Electrophoretic heterogeneity of lipopolysaccharides of Actinobacillus actinomycetemcomitans. Hoover, C.I. J. Dent. Res. (1988) [Pubmed]
  15. Electrophoretic analysis of the lipopolysaccharides of Bacteroides spp. Maskell, J.P. Antonie Van Leeuwenhoek (1994) [Pubmed]
  16. Evaluation of neurodegeneration in scrapie-infected animals by selective methods that detect cellular degeneration. Ye, X., Rountree, R., Scallet, A., Meeker, H.C., Carp, R.I. Brain Res. (2001) [Pubmed]
  17. Metabolomic and proteomic biomarkers for III-V semiconductors: chemical-specific porphyrinurias and proteinurias. Fowler, B.A., Conner, E.A., Yamauchi, H. Toxicol. Appl. Pharmacol. (2005) [Pubmed]
  18. Entorhinal cortex lesion in the mouse induces transsynaptic death of perforant path target neurons. Kovac, A.D., Kwidzinski, E., Heimrich, B., Bittigau, P., Deller, T., Nitsch, R., Bechmann, I. Brain Pathol. (2004) [Pubmed]
  19. Cell turnover in normal cycling human ovary. Funayama, Y., Sasano, H., Suzuki, T., Tamura, M., Fukaya, T., Yajima, A. J. Clin. Endocrinol. Metab. (1996) [Pubmed]
  20. Variation in the distribution of silver-staining nucleolar organizer regions on the chromosomes of the wild mouse, Mus musculus. Suzuki, H., Kurihara, Y., Kanehisa, T., Moriwaki, K. Mol. Biol. Evol. (1990) [Pubmed]
  21. Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein. Ochs, R.L., Busch, H. Exp. Cell Res. (1984) [Pubmed]
  22. Cd branding studies in a homologous Robertsonian 13;13 translocation. Romain, D.R., Columbano-Green, L., Sullivan, J., Smythe, R.H., Gebbie, O., Parfitt, R., Chapman, C. J. Med. Genet. (1982) [Pubmed]
  23. Cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii is a metalloprotease and digests the sodium perchlorate-insoluble component of cell wall. Matsuda, Y., Saito, T., Yamaguchi, T., Kawase, H. J. Biol. Chem. (1985) [Pubmed]
  24. Neuronal network disturbance after focal ischemia in rats. Kataoka, K., Hayakawa, T., Yamada, K., Mushiroi, T., Kuroda, R., Mogami, H. Stroke (1989) [Pubmed]
  25. Detection of fibrillarin in nucleolar remnants and the nucleolar matrix. Ochs, R.L., Smetana, K. Exp. Cell Res. (1991) [Pubmed]
  26. Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells. Jiang, X.S., Tang, L.Y., Cao, X.J., Zhou, H., Xia, Q.C., Wu, J.R., Zeng, R. Electrophoresis (2005) [Pubmed]
  27. Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting. King, S.M., Otter, T., Witman, G.B. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  28. High resolution two-dimensional gel electrophoresis of human erythrocyte membrane proteins. Copeland, B.R., Todd, S.A., Furlong, C.E. Am. J. Hum. Genet. (1982) [Pubmed]
  29. Demonstration of amyloid deposits and neurofibrillary changes in whole brain sections. Braak, H., Braak, E. Brain Pathol. (1991) [Pubmed]
  30. Rainbow's end: the quest for multiplexed fluorescence quantitative analysis in proteomics. Patton, W.F., Beechem, J.M. Current opinion in chemical biology. (2002) [Pubmed]
  31. Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities. Ito, M., Yamagata, T. J. Biol. Chem. (1989) [Pubmed]
  32. Nucleolar distribution of proteins B23 and nucleolin in mouse preimplantation embryos as visualized by immunoelectron microscopy. Biggiogera, M., Bürki, K., Kaufmann, S.H., Shaper, J.H., Gas, N., Amalric, F., Fakan, S. Development (1990) [Pubmed]
  33. Focal cellular origin and regulation of interstitial collagenase (matrix metalloproteinase-1) are related to menstrual breakdown in the human endometrium. Kokorine, I., Marbaix, E., Henriet, P., Okada, Y., Donnez, J., Eeckhout, Y., Courtoy, P.J. J. Cell. Sci. (1996) [Pubmed]
  34. The cyclin kinase inhibitor p21CIP1/WAF1 limits glomerular epithelial cell proliferation in experimental glomerulonephritis. Kim, Y.G., Alpers, C.E., Brugarolas, J., Johnson, R.J., Couser, W.G., Shankland, S.J. Kidney Int. (1999) [Pubmed]
  35. Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies. Jekabsons, M.B., Echtay, K.S., Brand, M.D. Biochem. J. (2002) [Pubmed]
  36. PAI-1 secretion and matrix deposition in human peritoneal mesothelial cell cultures: transcriptional regulation by TGF-beta 1. Rougier, J.P., Guia, S., Hagège, J., Nguyen, G., Ronco, P.M. Kidney Int. (1998) [Pubmed]
  37. Murine epidermal growth factor: heterogeneity on high resolution ion-exchange chromatography. Burgess, A.W., Lloyd, C.J., Nice, E.C. EMBO J. (1983) [Pubmed]
  38. The production and characterization of murine monoclonal antibodies to a DNA receptor on human leukocytes. Bennet, R.M., Hefeneider, S.H., Bakke, A., Merritt, M., Smith, C.A., Mourich, D., Heinrich, M.C. J. Immunol. (1988) [Pubmed]
  39. Purification of an active opioid-binding protein from bovine striatum. Gioannini, T.L., Howard, A.D., Hiller, J.M., Simon, E.J. J. Biol. Chem. (1985) [Pubmed]
  40. Nonradioactive arbitrarily primed polymerase chain reaction: a novel technique for detecting genetic defects in skin tumors. Vogt, T., Stolz, W., Landthaler, M., Rüschoff, J., Schlegel, J. J. Invest. Dermatol. (1996) [Pubmed]
  41. Biochemical characterization of mouse brain necdin. Maruyama, E. Biochem. J. (1996) [Pubmed]
 
WikiGenes - Universities