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Gene Review

HSP90AA1  -  heat shock protein 90kDa alpha (cytosolic)...

Sus scrofa

Synonyms: HSP90, Hspca
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Disease relevance of HSP90


High impact information on HSP90


Biological context of HSP90

  • We have isolated and sequenced cDNA clones encoding a 90-kDa heat shock protein (HSP90) from a porcine brain cDNA library [2].
  • Moreover, the deduced amino acid sequence of the porcine hsp90 cDNA was 99.7% identical to that of the human counterpart, with a difference of only three amino acids in a total of 733 residues [2].
  • HSP90 forms transient or stable complexes with several key proteins involved in signal transduction including protooncogenic protein kinases and nuclear receptors, it interacts with cellular structural elements such as actin-microfilament, tubulin-microtubule and intermediate filaments, and also exhibits conventional chaperone functions [10].
  • The aim of the present study was to further characterize the plausible novel function of HSP90 on sperm motility [11].
  • Hsp90 function is intimately linked to intrinsic ATP binding and hydrolysis activities, the latter of which is under the regulatory control of accessory factors [12].

Anatomical context of HSP90


Associations of HSP90 with chemical compounds

  • It has been reported that immunosuppressant cyclosporin A or FK506 binds to immunophilins in the cell and that these immunophilins make a complex with molecular chaperones HSP70 or HSP90 [15].
  • Geldanamycin, a HSP90 inhibitor, prevented the taxol-induced increase in eNOS activity [13].
  • Incubation of PAEC with nocodazole (50 microM) for 2-4 h induced a decrease in NO production, eNOS activity, and the amount of HSP90 binding to eNOS [13].
  • CONCLUSIONS: Taken together, these data indicate that AMPK activity is essential for estradiol-induced eNOS activation via the promotion of eNOS interaction with HSP90 [16].
  • However, inhibition of AMPK did not alter estradiol-induced eNOS phosphorylation at serine 1177 or threonine 495 but decreased eNOS interaction with HSP90 [16].

Other interactions of HSP90


Analytical, diagnostic and therapeutic context of HSP90

  • METHODS: Heat shock proteins (HSPs) were detected by immunoblotting with antibody against HSP90, HSP70, heat shock cognate protein 70, or HSP60 [18].
  • As determined by the molecular mass and isoelectric point on 2-dimensional gels and Western immunoblot with a specific 90 kDa heat shock protein (HSP90) monoclonal antibody, the unknown protein was identified as HSP90 [1].
  • MALDI mass spectra of HSP90 samples after chemical cross-linking showed a high percentage of alpha-alpha homodimers [10].
  • These results indicate that small-scale HPLC purification of HSP90 from porcine brain tissue can be readily accomplished, with high yield, using a convenient one-step purification method [19].
  • In this paper, the interactions of ATP with native hsp90 and its recombinant N-terminal (positions 1-221) and C-terminal (positions 446-728) domains were studied by isothermal titration calorimetry, scanning differential calorimetry, and fluorescence spectroscopy [7].


  1. Substantial decrease of heat shock protein 90 in ventricular tissues of two sudden-death pigs with hypertrophic cardiomyopathy. Lee, W.C., Lin, K.Y., Chiu, Y.T., Lin, J.H., Cheng, H.C., Huang, H.C., Yang, P.C., Liu, S.K., Mao, S.J. FASEB J. (1996) [Pubmed]
  2. Molecular cloning and characterization of porcine cDNA encoding a 90-kDa heat shock protein and its expression following hyperthermia. Huang, H., Lee, W.C., Lin, J.H., Jian, S.C., Mao, S.J., Yang, P.C., Huang, T.Y., Liu, Y.C. Gene (1999) [Pubmed]
  3. Role of calpain in hypoxic inhibition of nitric oxide synthase activity in pulmonary endothelial cells. Su, Y., Block, E.R. Am. J. Physiol. Lung Cell Mol. Physiol. (2000) [Pubmed]
  4. Liberation of the intramolecular interaction as the mechanism of heat-induced activation of HSP90 molecular chaperone. Tanaka, E., Nemoto, T.K., Ono, T. Eur. J. Biochem. (2001) [Pubmed]
  5. Purification and characterization of the 90-kDa heat-shock protein from mammalian tissues. Yonezawa, N., Nishida, E., Sakai, H., Koyasu, S., Matsuzaki, F., Iida, K., Yahara, I. Eur. J. Biochem. (1988) [Pubmed]
  6. Glutathione depletion impairs transcriptional activation of heat shock genes in primary cultures of guinea pig gastric mucosal cells. Rokutan, K., Hirakawa, T., Teshima, S., Honda, S., Kishi, K. J. Clin. Invest. (1996) [Pubmed]
  7. Binding of ATP to heat shock protein 90: evidence for an ATP-binding site in the C-terminal domain. Garnier, C., Lafitte, D., Tsvetkov, P.O., Barbier, P., Leclerc-Devin, J., Millot, J.M., Briand, C., Makarov, A.A., Catelli, M.G., Peyrot, V. J. Biol. Chem. (2002) [Pubmed]
  8. Heat-induced chaperone activity of HSP90. Yonehara, M., Minami, Y., Kawata, Y., Nagai, J., Yahara, I. J. Biol. Chem. (1996) [Pubmed]
  9. The 90-kDa heat shock protein, HSP90, binds and protects casein kinase II from self-aggregation and enhances its kinase activity. Miyata, Y., Yahara, I. J. Biol. Chem. (1992) [Pubmed]
  10. Phosphorylation and oligomerization states of native pig brain HSP90 studied by mass spectrometry. Garnier, C., Lafitte, D., Jorgensen, T.J., Jensen, O.N., Briand, C., Peyrot, V. Eur. J. Biochem. (2001) [Pubmed]
  11. The decline of porcine sperm motility by geldanamycin, a specific inhibitor of heat-shock protein 90 (HSP90). Huang, S.Y., Kuo, Y.H., Tsou, H.L., Lee, Y.P., King, Y.T., Huang, H.C., Yang, P.C., Lee, W.C. Theriogenology (2000) [Pubmed]
  12. Adenosine nucleotides and the regulation of GRP94-client protein interactions. Rosser, M.F., Trotta, B.M., Marshall, M.R., Berwin, B., Nicchitta, C.V. Biochemistry (2004) [Pubmed]
  13. Microtubule-active agents modify nitric oxide production in pulmonary artery endothelial cells. Su, Y., Zharikov, S.I., Block, E.R. Am. J. Physiol. Lung Cell Mol. Physiol. (2002) [Pubmed]
  14. HSP90 and Akt modulate Ang-1-induced angiogenesis via NO in coronary artery endothelium. Chen, J.X., Lawrence, M.L., Cunningham, G., Christman, B.W., Meyrick, B. J. Appl. Physiol. (2004) [Pubmed]
  15. Mammalian HSP60 is a major target for an immunosuppressant mizoribine. Itoh, H., Komatsuda, A., Wakui, H., Miura, A.B., Tashima, Y. J. Biol. Chem. (1999) [Pubmed]
  16. Estradiol-mediated endothelial nitric oxide synthase association with heat shock protein 90 requires adenosine monophosphate-dependent protein kinase. Schulz, E., Anter, E., Zou, M.H., Keaney, J.F. Circulation (2005) [Pubmed]
  17. Impaired NO signaling in small pulmonary arteries of chronically hypoxic newborn piglets. Fike, C.D., Aschner, J.L., Zhang, Y., Kaplowitz, M.R. Am. J. Physiol. Lung Cell Mol. Physiol. (2004) [Pubmed]
  18. Geranylgeranylacetone induces heat shock proteins in cultured guinea pig gastric mucosal cells and rat gastric mucosa. Hirakawa, T., Rokutan, K., Nikawa, T., Kishi, K. Gastroenterology (1996) [Pubmed]
  19. One-step HPLC purification procedure for porcine brain 90-kDa heat shock protein. Chu, L.F., Lee, W.C., Yang, P.C., Chu, R., Huang, T.Y., Mao, S.J. Protein Expr. Purif. (1997) [Pubmed]
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