The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

High-sensitivity detection and postsource decay of 2-aminopyridine-derivatized oligosaccharides with matrix-assisted laser desorption/ionization mass spectrometry.

The sensitivities of oligosaccharide derivatives in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) were compared using two matrixes, 2,5-dihydroxybenzoic acid (DHBA) and alpha-cyano-4-hydroxycinnamic acid (CHCA). For this purpose, maltopentaose was tagged with 2-aminopyridine (PA), 4-aminobenzoic acid ethyl ester (ABEE), and trimethyl-(p-aminophenyl)ammonium chloride (TMAPA). DHBA was more advantageous for enhancement than CHCA. Among the derivatives, the sensitivity with the PA-tagged maltopentaose showed a 100-fold improvement over the native one with DHBA as a matrix, while the oligesaccharide derivatized with ABEE and TMAPA gave 30- and 10-fold increases in sensitivity over the underivatized one. To obtain structural information from these derivatized oligosaccharides, postsource decay (PSD) during flight in the field-free drift in MALDI-TOFMS was measured. Predictable and reproducible fragmentation patterns could be obtained in all cases. Furthermore, we found matrix-dependence fragmentation with the PA-labeled oligosaccharide. With CHCA, a simple spectrum ascribable to Y series ions was obtained. On the other hand, both B and Y series ions were clearly observed in the DHBA case. The results demonstrate the usefulness of derivatives for sensitive analysis of oligosaccharides with MALDI.[1]

References

 
WikiGenes - Universities