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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Bovine cementum extract influences murine dental follicle cells in vitro.

This study evaluated the attachment, chemoattractive, proliferative and mineralization inductive potential of a bovine cementum extract ( CPE) on newborn murine dental follicle cells (MDFC) in vitro. Cementum extract was partially purified by DEAE-cellulose chromatography. A band representing an M(r) of 55,000 was excised from the gel and the protein(s) were electroeluted. Attachment assays revealed that CPE (1.0 microgram/ml) promoted MDFC attachment by 96% in comparison with collagen type I (5 micrograms/ml), and was five-fold greater compared with serum-free media (SFM), (P < 0.05). Between 1 and 5 days CPE at 1.0 microgram/ml and collagen type I at 5 micrograms/ml sustained more than 75% attachment and spreading of MDFC when compared to SFM (P < 0.05). Contrary to other reports, fibronectin (0.5 microgram/ml) was more potent than CPE in promoting MDFC chemoattraction (P < 0.05). MDFC proliferation was stimulated by CPE (0.125 microgram/ml), but this response was elicited only when CPE was used together with 10% FBS (37.3%) or 0.2% FBS (76%) (P < 0.05). Alkaline phosphatase expression by MDFC was increased by CPE (1.0 microgram/ml), in comparison to the control. Calcium deposits were detected by von Kossa staining in 14-day MDFC cultures treated with CPE. Nodule formation and its mineralization in long-term MDFC cultures were induced by CPE (1.0 microgram/ml). Molecule(s) contained in CPE appear to regulate various biological activities in MDFC, indicating that CPE could play a key role in selecting progenitor cells required for the process of cementogenesis during development.[1]

References

  1. Bovine cementum extract influences murine dental follicle cells in vitro. Arzate, H., Aguilar-Mendoza, M.E., Esponda Aguilar, C., Portilla Robertson, J. Arch. Med. Res. (1997) [Pubmed]
 
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