cDNA cloning and expression analysis of the murine ribonuclease L inhibitor.
The 2-5A/RNase L system is one of the pathways induced by interferon ( IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor ( RLI). Human RLI (Hu- RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI ( Mu- RLI). Hu- RLI and Mu- RLI protein have 98% amino acid identity. Mu- RLI is functionally homologous to Hu- RLI, and all the structural features and amino acid sequence motifs of Hu- RLI are conserved in Mu- RLI. Moreover, reticulocyte lysate translated Mu- RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu- RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation.[1]References
- cDNA cloning and expression analysis of the murine ribonuclease L inhibitor. Benoit De Coignac, A., Bisbal, C., Lebleu, B., Salehzada, T. Gene (1998) [Pubmed]
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