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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A barbiturate screening assay for the Abbott AxSYM analyzer.

A fluorescence polarization immunoassay for barbiturates on the Abbott AxSYM analyzer is described. The assay displayed dilution linearity up to 1200 ng/mL; coefficients of variation varied from 5.96 to 8.61%; recovery varied from 94.9 to 105.3%; and sensitivity was less than 40 ng/mL. Good correlation between the standard six- and factory two-point calibration methods was observed. The Immunoassay demonstrated good cross-reactivity to several commonly prescribed barbiturates; low cross-reactivity with structurally similar compounds; low interference from endogenous substances, dyes, preservatives, and several commonly available adulterants; and good correlation with the TDx Barbiturate Urine assay.[1]

References

  1. A barbiturate screening assay for the Abbott AxSYM analyzer. Adamczyk, M., Douglas, J., Grote, J., Harrington, C.A. Journal of analytical toxicology. (1998) [Pubmed]
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