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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Hammer, E. et al.

Hammer, Krowas, Schäfer, Specht, Francke, Schauer,

Isolation and characterization of a dibenzofuran-degrading yeast: identification of oxidation and ring cleavage products.

We characterized the ability of a yeast to cleave the aromatic structure of the dioxin-like compound dibenzofuran. The yeast strain was isolated from a dioxin-contaminated soil sample and identified as Trichosporon mucoides. During incubation of glucose-pregrown cells with dibenzofuran, six major metabolites were detected by high-performance liquid chromatography. The formation of four different monohydroxylated dibenzofurans was proven by comparison of analytical data ( gas chromatography-mass spectrometry) with that for authentic standards. Further oxidation produced 2, 3-dihydroxydibenzofuran and its ring cleavage product 2-(1-carboxy methylidene)-2,3-dihydrobenzo[b]furanylidene glycolic acid, which were characterized by mass spectrometry and 1H nuclear magnetic resonance spectroscopy. These two metabolites are derived from 2-hydroxydibenzofuran and 3-hydroxydibenzofuran, as shown by incubation experiments using these monohydroxylated dibenzofurans as substrates.[1]

References

Isolation and characterization of a dibenzofuran-degrading yeast: identification of oxidation and ring cleavage products. Hammer, E., Krowas, D., Schäfer, A., Specht, M., Francke, W., Schauer, F. Appl. Environ. Microbiol. (1998) [Pubmed]

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