Disease relevance of IGKV1-33

• Most (80%) of these E. coli K1 strains were serotyped as O7, O18, O83, or were autoagglutinable [1].
• Monoclonal IgM specific for the O18 antigen conferred passive protection to 1-week-old rats against bacteremia and killing after oral challenge with O18:K1 Escherichia coli [2].
• Monoclonal antibodies (MAbs) reactive with selected O antigen serotypes of Escherichia coli (O18) and Salmonella typhimurium (O9, 12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals [3].
• To study antibody-mediated protection against Escherichia coli peritonitis in BALB/c mice, monoclonal antibodies (MAbs) were generated against the capsule (K5) and the lipopolysaccharide (O18) of E. coli [4].
• The O18 lipopolysaccharide contains a phosphocholine substitute, which makes it unique among Proteus strains [5].

High impact information on IGKV1-33

• V kappa I antibodies appear to be products of three newly sequenced V kappa I genes, O8, O18, and L11, that are reported here [6].
• A single mutation in the gene that codes for UDP N-acetylgalactosamine 4-epimerase (gne) confers the O(-) phenotype (LPS without O-antigen molecules) on a strain in serotypes O18 and O34, but not in serotypes O1 and O2 [7].
• Serum obtained after immunization with an O18 polysaccharide-toxin A conjugate vaccine was evaluated for the estimation of protective levels of anti-O-specific lipopolysaccharide (LPS) immunoglobulin G (IgG) antibody against bacteremia and death caused by a homologous serotype of Escherichia coli K1 strains [8].
• Colonic resident strains expressed P fimbriae, adhered to colonic epithelial cells via a mannose-resistant mechanism, and expressed the uropathogenic serotypes O1, O2, O6, O7, O18, O25, or O75 more often than did the transient strains, which were often nontypeable [9].
• O1, O2, O6, O7, O18, O25, and rough-type lipopolysaccharide accounted for 40% of all isolates [10].

Chemical compound and disease context of IGKV1-33

• K antigen did not affect serum sensitivity, but E. coli strains of O types O18, O2, O4, and O7 were more serum-resistant than other E. coli [11].
• The variant antibodies retained the antigen specificity of the parental IgM for the O18 (lipopolysaccharide) antigen of Escherichia coli [12].
• The comparison of hemolysin production with possession of the aerobactin system in virulent clones of E. coli K1 strains showed that all of the aerobactin-producing strains from the O18 MP9 and O7 MP3 clonal groups did not synthesize hemolysin, whereas 11 of 12 aerobactin-nonproducing O18 MP6 isolates were hemolytic [13].
• Nontoxic, serologically reactive O polysaccharide was derived from Escherichia coli O18 lipopolysaccharide by acid hydrolysis, extraction with organic solvents, and gel filtration chromatography [14].
• Virulence factors of Escherichia coli. II. Antigens O4, O6 and O18, haemolysin production and mannose resistant haemagglutinating capacity are closely associated [15].

Biological context of IGKV1-33

• The O8 and O18 genes encode identical amino acid sequences [6].
• Both conjugates engendered an immunoglobulin G antibody response in rabbits that recognized native O18 lipopolysaccharide [14].
• However, a hybridization probe derived from a sequence adjacent to the hlyC-proximal end of the plasmid pHly 152-encoded hly determinant hybridizes with several additional chromosomal bands in hemolytic O18 and O6 E. coli strains and even in E. coli K-12 [16].
• Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea [17].
• S fimbriae genotypes were observed more frequently in septic (25%) and urinary (12%) isolates of E. coli than in faecal and water isolates (1%) and often occurred together with O2, O6, O18 and O83 antigens [18].

Anatomical context of IGKV1-33

• Efficient lysis of sheep erythrocytes coated with the O18 antigen was observed with IgM and all IgG subclasses, but no lysis was triggered by IgE or IgA [12].
• Interleukin-8 response in cells from the human urinary tract induced by lipopolysaccharides of Proteus mirabilis O3 and O18 [5].
• Interestingly, 3d279d is frequently expressed by B cells stimulated with staphylococcal enterotoxin; and O18 is the main gene employed by the Vk1 IgG antibodies against Haemophilus influenzae [19].
• In this study, Acanthamoeba castellanii (a keratitis isolate belonging to the T4 genotype) was used and its interactions with Escherichia coli (strain K1, a cerebrospinal fluid isolate from a meningitis patient, O18 : K1 : H7, and a K-12 laboratory strain, HB101) were studied [20].

Associations of IGKV1-33 with chemical compounds

• Among strains of the eight O-types most frequently causing such infections at this hospital, O4, O9, and O18 had a high incidence of multiple resistance (35, 22, and 19%, respectively); O2 and O6 had a intermediate incidence (14 and 11%, respectively); and O7, O1, and O75 had a low incidence (8, 6, and less than 3%, respectively) [21].
• Specific protection of the pups was also achieved by immunizing the pregnant rats with purified O18 lipopolysaccharide [2].
• In contrast, the aerobactin system was not detected in strains from the O18 MP6 clone [13].
• Mass spectrometric analysis enabled detection of isotopic O18 enrichment at parts per million levels in blood and in breath CO2 [22].
• Pathogenesis studies in chickens and colostrum-deprived calves revealed that the loss of virulence exhibited by the forms of the MW strain that lacked O18, K1 and ColV and by the NTG-derived form was associated with decreased ability to invade the body [23].

Analytical, diagnostic and therapeutic context of IGKV1-33

• Sera from normal healthy human adults and infants, as well as sera from mice, rabbits, and guinea pigs, were examined by immunoblotting for naturally occurring antibodies reacting with outer membrane proteins of two Escherichia coli strains, O111 and O18 [24].
• RESULTS: Sequence analysis of CG1 revealed that its heavy and light chain V regions were respectively most homologous to the 3d279d VH4 and the O18 Vk1 genes [19].

References