The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

NCF4  -  neutrophil cytosolic factor 4, 40kDa

Homo sapiens

Synonyms: NCF, NCF-4, Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, P40PHOX, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of NCF4


High impact information on NCF4

  • The antibody against the COOH terminus of p67phox was able to communoprecipitate p40phox in conjunction with p67phox itself as was expected [6].
  • Involvement of p40phox in activation of phagocyte NADPH oxidase through association of its carboxyl-terminal, but not its amino-terminal, with p67phox [6].
  • Similar results were found regarding their effects on the O2-generating ability of cytosol in a cell-free activation system, i.e., inhibition was noted with the COOH terminus antibody but not with that for the NH2 terminus of p40phox [6].
  • These results provide the first demonstration that p40phox is practically involved in the activation of NADPH oxidase through the association of its COOH-terminal, but not its NH2-terminal, with p67phox [6].
  • A heat-stable neutrophil chemotactic factor (NCF) has been identified in the serum of 13 atopic asthmatic subjects after treadmill exercise [7].

Chemical compound and disease context of NCF4

  • Biochemical analysis demonstrated that several kinds of NCF, including those derived from the complement component C5 and alveolar macrophages, were present in the BAL fluid from patients with CAD and respiratory infections [8].

Biological context of NCF4


Anatomical context of NCF4


Associations of NCF4 with chemical compounds

  • Chronic ACT was associated with a variant of the NAD(P)H oxidase subunit NCF4 (rs1883112, -212A-->G; symbols with right-pointing arrows, as edited?' odds ratio [OR], 2.5; 95% CI, 1.3 to 5.0) [16].
  • The entire coding region of p40phox was shown to interact with TRX both in assays of histidine prototrophy and beta-galactosidase activity; in contrast, no interaction was observed with substituted mutant TRX (C32S/C35S), which lacks reducing activity [10].
  • Although actin has been reported to be involved in O2- generation, the p40phox profile did not correspond to that of actin [1].
  • The tight association between p40phox and p67phox was not affected by treatment with a mixture of deoxycholate and Nonidet P-40, until subjected to SDS-PAGE [1].
  • Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex [17].

Physical interactions of NCF4

  • These results showed that p40phox interacts with TRX and indicated the possibility of TRX-dependent regulation of phagocyte oxidase activity [10].
  • Binding studies revealed that coronin attaches to the C-terminal half of p40phox, a binding partner of p67phox [18].
  • CONCLUSIONS: In the resting cell, the Pro-rich motif of p47phox interacts with the SH3 domain of p40phox, which in turn associates with p67phox [19].

Enzymatic interactions of NCF4


Other interactions of NCF4


Analytical, diagnostic and therapeutic context of NCF4


  1. A novel cytosolic component, p40phox, of respiratory burst oxidase associates with p67phox and is absent in patients with chronic granulomatous disease who lack p67phox. Tsunawaki, S., Mizunari, H., Nagata, M., Tatsuzawa, O., Kuratsuji, T. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  2. Exercise-induced release of histamine and neutrophil chemotactic factor in atopic asthmatics. Lee, T.H., Brown, M.J., Nagy, L., Causon, R., Walport, M.J., Kay, A.B. J. Allergy Clin. Immunol. (1982) [Pubmed]
  3. NF-kappaB activation and sustained IL-8 gene expression in primary cultures of cystic fibrosis airway epithelial cells stimulated with Pseudomonas aeruginosa. Joseph, T., Look, D., Ferkol, T. Am. J. Physiol. Lung Cell Mol. Physiol. (2005) [Pubmed]
  4. Neutrophil chemotactic factor release and neutrophil alveolitis in asbestos-exposed individuals. Hayes, A.A., Rose, A.H., Musk, A.W., Robinson, B.W. Chest (1988) [Pubmed]
  5. Fibroblasts isolated from human pterygia exhibit altered lipid metabolism characteristics. Peiretti, E., Dessì, S., Mulas, M.F., Abete, C., Galantuomo, M.S., Fossarello, M. Exp. Eye Res. (2006) [Pubmed]
  6. Involvement of p40phox in activation of phagocyte NADPH oxidase through association of its carboxyl-terminal, but not its amino-terminal, with p67phox. Tsunawaki, S., Kagara, S., Yoshikawa, K., Yoshida, L.S., Kuratsuji, T., Namiki, H. J. Exp. Med. (1996) [Pubmed]
  7. Identification and partial characterization of an exercise-induced neutrophil chemotactic factor in bronchial asthma. Lee, T.H., Nagy, L., Nagakura, T., Walport, M.J., Kay, A.B. J. Clin. Invest. (1982) [Pubmed]
  8. Neutrophil chemotactic factors in the respiratory tract of patients with chronic airway diseases or idiopathic pulmonary fibrosis. Ozaki, T., Hayashi, H., Tani, K., Ogushi, F., Yasuoka, S., Ogura, T. Am. Rev. Respir. Dis. (1992) [Pubmed]
  9. The Ku70 autoantigen interacts with p40phox in B lymphocytes. Grandvaux, N., Grizot, S., Vignais, P.V., Dagher, M.C. J. Cell. Sci. (1999) [Pubmed]
  10. Demonstration of the interaction of thioredoxin with p40phox, a phagocyte oxidase component, using a yeast two-hybrid system. Nishiyama, A., Ohno, T., Iwata, S., Matsui, M., Hirota, K., Masutani, H., Nakamura, H., Yodoi, J. Immunol. Lett. (1999) [Pubmed]
  11. Rac1 disrupts p67phox/p40phox binding: a novel role for Rac in NADPH oxidase activation. Rinckel, L.A., Faris, S.L., Hitt, N.D., Kleinberg, M.E. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  12. The superoxide-generating NADPH oxidase: structural aspects and activation mechanism. Vignais, P.V. Cell. Mol. Life Sci. (2002) [Pubmed]
  13. The 40-kDa component of the phagocyte NADPH oxidase (p40phox) is phosphorylated during activation in differentiated HL60 cells. Fuchs, A., Bouin, A.P., Rabilloud, T., Vignais, P.V. Eur. J. Biochem. (1997) [Pubmed]
  14. Mechanisms of NADPH oxidase activation: translocation of p40phox, Rac1 and Rac2 from the cytosol to the membranes in human neutrophils lacking p47phox or p67phox. Dusi, S., Donini, M., Rossi, F. Biochem. J. (1996) [Pubmed]
  15. A constitutive NADPH oxidase-like system containing gp91phox homologs in human keratinocytes. Chamulitrat, W., Stremmel, W., Kawahara, T., Rokutan, K., Fujii, H., Wingler, K., Schmidt, H.H., Schmidt, R. J. Invest. Dermatol. (2004) [Pubmed]
  16. NAD(P)H oxidase and multidrug resistance protein genetic polymorphisms are associated with doxorubicin-induced cardiotoxicity. Wojnowski, L., Kulle, B., Schirmer, M., Schlüter, G., Schmidt, A., Rosenberger, A., Vonhof, S., Bickeböller, H., Toliat, M.R., Suk, E.K., Tzvetkov, M., Kruger, A., Seifert, S., Kloess, M., Hahn, H., Loeffler, M., Nürnberg, P., Pfreundschuh, M., Trümper, L., Brockmöller, J., Hasenfuss, G. Circulation (2005) [Pubmed]
  17. Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex. Zhan, S., Vazquez, N., Zhan, S., Wientjes, F.B., Budarf, M.L., Schrock, E., Ried, T., Green, E.D., Chanock, S.J. Blood (1996) [Pubmed]
  18. Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils. Grogan, A., Reeves, E., Keep, N., Wientjes, F., Totty, N.F., Burlingame, A.L., Hsuan, J.J., Segal, A.W. J. Cell. Sci. (1997) [Pubmed]
  19. SH3 domain-mediated interactions involving the phox components of the NADPH oxidase. Wilson, L., Butcher, C., Finan, P., Kellie, S. Inflamm. Res. (1997) [Pubmed]
  20. Phosphorylation of p40-phox during activation of neutrophil NADPH oxidase. Someya, A., Nunoi, H., Hasebe, T., Nagaoka, I. J. Leukoc. Biol. (1999) [Pubmed]
  21. The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain. Wientjes, F.B., Reeves, E.P., Soskic, V., Furthmayr, H., Segal, A.W. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  22. Characteristics of NADPH oxidase genes (Nox2, p22, p47, and p67) and Nox4 gene expressed in blood cells of juvenile Ciona intestinalis. Inoue, Y., Ogasawara, M., Moroi, T., Satake, M., Azumi, K., Moritomo, T., Nakanishi, T. Immunogenetics (2005) [Pubmed]
  23. Identification of a splice variant mRNA of p40phox, an NADPH oxidase component of phagocytes. Hasebe, T., Someya, A., Nagaoka, I. FEBS Lett. (1999) [Pubmed]
  24. P40phox associates with the neutrophil Triton X-100-insoluble cytoskeletal fraction and PMA-activated membrane skeleton: a comparative study with P67phox and P47phox. El Benna, J., Dang, P.M., Andrieu, V., Vergnaud, S., Dewas, C., Cachia, O., Fay, M., Morel, F., Chollet-Martin, S., Hakim, J., Gougerot-Pocidalo, M.A. J. Leukoc. Biol. (1999) [Pubmed]
  25. The PC motif: a novel and evolutionarily conserved sequence involved in interaction between p40phox and p67phox, SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase. Nakamura, R., Sumimoto, H., Mizuki, K., Hata, K., Ago, T., Kitajima, S., Takeshige, K., Sakaki, Y., Ito, T. Eur. J. Biochem. (1998) [Pubmed]
WikiGenes - Universities