Disease relevance of ZNF35

• We have expressed the ZNF35 protein from E. coli and have employed a Southwestern-polymerase chain reaction method using random oligonucleotides to identify its high-affinity binding site [1].
• By in situ hybridization experiments and analysis of interspecific somatic cell hybrids we mapped the HF.10 gene to 3p21-22, a chromosome region frequently involved in karyotypic rearrangements associated with lung and renal cancer [2].
• Intratumoral injection of herpes simplex virus HF10 in recurrent breast cancer [3].
• Pilot Study of Oncolytic Viral Therapy Using Mutant Herpes Simplex Virus (HF10) Against Recurrent Metastatic Breast Cancer [4].
• METHODS: A pilot study using HF10 was initiated in six patients with cutaneous or subcutaneous metastases from breast cancer [4].

High impact information on ZNF35

• The region surrounding the ZNF35 zinc finger protein gene on 3p21 is of particular interest, as this region of chromosome 3 is frequently involved in rearrangements and/or deletions associated with various human tumors including lung and renal carcinoma [5].
• We have analyzed yeast artificial chromosomes (YACs), identified by PCR screening, using oligonucleotides derived from the ZNF35 gene [5].
• CONCLUSIONS: This pilot study found HF10 to be safe and effective against metastatic breast cancer [4].
• A comparison between pimonidazole binding expressed as the fraction of fields at the highest score and HF10 showed a trend for the most well-oxygenated tumors having a low fraction of fields; however, the correlation did not reach statistical significance (p = 0.43, r = 0.165; Spearman's rank correlation test) [6].
• The ZNF35 human zinc finger gene encodes a sequence-specific DNA-binding protein [1].

Biological context of ZNF35

• These features suggest that ZNF35 is a site-specific DNA-binding protein involved in the regulation of gene expression [1].
• The core sequence for the ZNF35 protein-binding site is 5'-C/GC/GAAG/TA-3' [1].
• We report the structural and functional characterization of the HF.10 zinc finger gene (ZNF35) in normal human cells, as well as a processed pseudogene [7].
• The HF.10 gene spans about 13 kb and it is interrupted by three introns [7].
• Hybridization of the HF.10 gene and the HF.10 pseudogene DNA probes to metaphases from a small cell lung carcinoma cell line with the 3p deletion revealed that both loci are part of the deleted chromosome region [7].

Anatomical context of ZNF35

• Using transient CAT assay in cotransfection experiments in cultured cells, we have determined that the HF.10 finger protein is a transcriptional transactivator [7].
• One of these finger genes, HF.10, is expressed at low levels in a variety of human tissues and is down-regulated during the in vitro terminal differentiation of human leukemic myeloid cell lines [2].
• This study shows that the ZFHO penetration ability of human spermatozoa with poor motility can be improved by using HTF medium as compared with the use of HF-10 medium [8].
• Forty semen specimens were each divided into two aliquots; one aliquot was washed with and incubated in HF-10 medium and the other in HTF medium [8].
• A large number of inflammatory cells infiltrated into the bladder mucosa at 3 days after HF10 treatment in the preimmunized mice [9].

Associations of ZNF35 with chemical compounds

• CONCLUSIONS: Our data suggest that vitamin E and HF-10 protect against the reactive oxygen species-mediated damage on spermatozoa [10].

Other interactions of ZNF35

• The genomic region surrounding HF.10 exon 1 contains a CpG island and acts as a promoter in vitro [7].
• The penetration ability of human spermatozoa on zona-free hamster ovum when using Ham's F-10 (HF-10) medium was compared with that when using a medium based on the composition of human tubal fluid [8].

Analytical, diagnostic and therapeutic context of ZNF35

• PFGE and Southern blot/hybridization analysis revealed that the clones cover 750-kb including the ZNF35 gene [5].
• Restriction enzyme mapping and partial nucleotide sequencing of the HF.10 pseudogene indicated that it has arisen by retroposition of spliced HF.10 mRNA [7].
• CONCLUSIONS: HF10 demonstrated antitumor effects in our animal model [11].

References