Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

dG-8-Aaf N-[2-amino-9-[(4S,5R)-4- hydroxy-5...

Synonyms: AC1L2XWQ, AC1Q6PDI, AR-1H4256, 37819-60-6, Acetylaminofluorene-dG, ...

Yamasaki, H. et al., Kriek, E. et al., Gupta, P.K. et al., Moriya, M. et al., Van Der Laken, C.J. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Acetylaminofluorene-dG

The specificity of N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene (G-8-AAF) adducts in double-stranded DNAs from M13mp8 and M13mp9 bacteriophage was determined following transfection of modified DNA with multiple adducts into competent JM103 cells [1].

High impact information on Acetylaminofluorene-dG

Mutagenic specificity of 2-acetylaminofluorene (AAF) has been established in mammalian cells and several strains of bacteria by using a shuttle plasmid vector containing a single N-(deoxyguanosin-8-yl)acetylaminofluorene (C8-dG-AAF) adduct [2].
Hydrolysis of the [14C]-N-acetyl-2-aminofluorene-modified DNA followed by Sephadex LH-20 column chromatography showed that 85% of the DNA-bound [14C]-N-acetyl-2-aminofluorene was N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene and 15% was 3-(deoxyguanosin-N2-yl)-N-acetyl-2-aminofluorene [3].
Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase zeta is stimulated by yeast Rev1 protein [4].
Highly specific antibodies were obtained (affinity constant, 6.0 X 10(9) l/mol) and have been employed in a sensitive radioimmunoassay to determine N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene directly in enzymatic hydrolysates of DNA modified with N-acetoxy-N-acetyl-2-aminofluorene [5].

Analytical, diagnostic and therapeutic context of Acetylaminofluorene-dG

Measurement of O6-ethyldeoxyguanosine and N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene in DNA by high-sensitive enzyme immunoassays [6].

References

Induction of mutations by N-acetoxy-N-acetyl-2-aminofluorene modified M13 viral DNA. Gupta, P.K., Pandrangi, R.G., Lee, M.S., King, C.M. Carcinogenesis (1991) [Pubmed]
Targeted mutations induced by a single acetylaminofluorene DNA adduct in mammalian cells and bacteria. Moriya, M., Takeshita, M., Johnson, F., Peden, K., Will, S., Grollman, A.P. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
Differential excision from DNA of the C-8 and N2 guanosine adducts of N-acetyl-2-aminofluorene by single strand-specific endonucleases. Yamasaki, H., Pulkrabek, P., Grunberger, D., Weinstein, I.B. Cancer Res. (1977) [Pubmed]
Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase zeta is stimulated by yeast Rev1 protein. Guo, D., Xie, Z., Shen, H., Zhao, B., Wang, Z. Nucleic Acids Res. (2004) [Pubmed]
Immunological detection and quantification of the reaction products of 2-acetylaminofluorene with guanine in DNA. Kriek, E., Van der Laken, C.J., Welling, M., Nagel, J. IARC Sci. Publ. (1982) [Pubmed]
Measurement of O6-ethyldeoxyguanosine and N-(deoxyguanosin-8-yl)-N-acetyl-2-aminofluorene in DNA by high-sensitive enzyme immunoassays. Van Der Laken, C.J., Hagenaars, A.M., Hermsen, G., Kriek, E., Kuipers, A.J., Nagel, J., Scherer, E., Welling, M. Carcinogenesis (1982) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.