Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

AGN-PC-00GCKS [5-(6-aminopurin-9-yl)-4- hydroxy-2...

Synonyms: CHEMBL416789, CHEBI:156223, CTK8G5038, AC1L18F4, adenosine 3',5'-bis(dihydrogen phosphate)

Lee, Y.C. et al., McAllister, K.A. et al., Kramer, P.R. et al., AbdelRaheim, S.R. et al., Lee, Y.C. et al.

AbdelRaheim, McLennan,

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High impact information on phosphoadenosine phosphate

Further analysis of the inhibition kinetics of the product (3',5'-ADP) suggested that it is competitive with respect to CoA but mixed (competitive and noncompetitive) with respect to apo-ACP [1].
It is not yet known whether 3',5'-ADP acts allosterically or contributes directly to the structure of the steroid binding site [2].
Affinity chromatography of a partially purified preparation on agarose hexane 3',5'-ADP produces enzyme of high specific activity (351 units/mg) [3].
An interesting finding was that, in addition to EST activity, gpEST bound estradiol with a high affinity [dissociation constant (Kd) 10 nM]; furthermore, the binding activity was absolutely dependent on the cofactor, adenosine-3',5'-diphosphate (3',5'-ADP) [4].
The products of CoA hydrolysis were 3',5'-ADP and 4'-phosphopantetheine with Km and kcat values of 220 microM and 13.8 s(-1) respectively [5].

References

Biochemical and molecular analyses of the Streptococcus pneumoniae acyl carrier protein synthase, an enzyme essential for fatty acid biosynthesis. McAllister, K.A., Peery, R.B., Meier, T.I., Fischl, A.S., Zhao, G. J. Biol. Chem. (2000) [Pubmed]
Purification and identification of the heat-stable factor required for pregnenolone-binding protein activity. Evidence that the factor is adenosine 3',5'-diphosphate. Lee, Y.C., Oeda, T., Driscoll, W.J., Fales, H.M., Strott, C.A. J. Biol. Chem. (1992) [Pubmed]
Purification and characterization of avian liver 3-hydroxy-3-methylglutaryl coenzyme A lyase. Kramer, P.R., Miziorko, H.M. J. Biol. Chem. (1980) [Pubmed]
Structural and functional characterization of estrogen sulfotransferase isoforms: distinct catalytic and high affinity binding activities. Lee, Y.C., Komatsu, K., Driscoll, W.J., Strott, C.A. Mol. Endocrinol. (1994) [Pubmed]
The Caenorhabditis elegans Y87G2A.14 Nudix hydrolase is a peroxisomal coenzyme A diphosphatase. AbdelRaheim, S.R., McLennan, A.G. BMC Biochem. (2002) [Pubmed]

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