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Gene Review:

ompT - outer membrane protease

Escherichia coli CFT073

Malik, A. et al., Wu, C.F. et al., Hwang, B.Y. et al., Maurer, J. et al., Matsuo, E. et al.

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Disease relevance of ompT

Efficient presentation of the passenger domains was demonstrated in the outer membrane protease T-deficient (ompT) strain E. coli UT5600 and the ompT dsbA double mutant JK321 [1].

High impact information on ompT

Accordingly, we show that the presence of an F' episome results in increased resistance to the antimicrobial peptide protamine both in ompT mutants and in wild-type E. coli cells [2].
Here we report that SecY cleavage occurs even in cell extracts from ompT-disrupted cells, if they carry an F plasmid derivative [3].
To test the utility of this microarray device to detect genetically engineered bacteria, E. coli BL21 (a B strain derivative with antibiotic resistance gene, ampR) and E. coli JM107 (a K12 strain derivative lacking the gene ompT) were also employed [4].
After fusion to pelB, dsbA or ompT signal peptides no recombinant product could be obtained in the periplasm using the T7 promoter [5].

References

Autodisplay: one-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli. Maurer, J., Jose, J., Meyer, T.F. J. Bacteriol. (1997) [Pubmed]
Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpP. Hwang, B.Y., Varadarajan, N., Li, H., Rodriguez, S., Iverson, B.L., Georgiou, G. J. Bacteriol. (2007) [Pubmed]
The plasmid F OmpP protease, a homologue of OmpT, as a potential obstacle to E. coli-based protein production. Matsuo, E., Sampei, G., Mizobuchi, K., Ito, K. FEBS Lett. (1999) [Pubmed]
DNA microarray for discrimination between pathogenic 0157:H7 EDL933 and non-pathogenic Escherichia coli strains. Wu, C.F., Valdes, J.J., Bentley, W.E., Sekowski, J.W. Biosensors & bioelectronics. (2003) [Pubmed]
A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm. Malik, A., Rudolph, R., Söhling, B. Protein Expr. Purif. (2006) [Pubmed]

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