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Gene Review

infB  -  translation initiation factor IF-2

Escherichia coli CFT073

 
 
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Disease relevance of infB

 

High impact information on infB

  • A DNA fragment containing the entire infB gene was cloned into three plasmid vectors and the resulting recombinant DNAs were used as templates in assays containing fMet-tRNA and various labelled aminoacyl-tRNAs.(ABSTRACT TRUNCATED AT 250 WORDS)[3]
  • The N-terminal amino acid sequences are completely different, but match perfectly the DNA sequences at the beginning of the infB open reading frame and an in-phase region 471 bp downstream [3].
  • Here we report the topographical localization of translation initiation factor IF2 on the 70S ribosome determined by base-specific chemical probing [4].
  • Four-factor recombination analysis positioned the infB gene at 145 degrees on the B. subtilis chromosome, between the polC and spcB loci [5].
 

Biological context of infB

  • The gene infB was cloned into the thermo-inducible runaway plasmid pCP40 [1] and subsequently transformed into the E coli strain C600[pcI857] [6].

References

  1. Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli. Laursen, B.S., Siwanowicz, I., Larigauderie, G., Hedegaard, J., Ito, K., Nakamura, Y., Kenney, J.M., Mortensen, K.K., Sperling-Petersen, H.U. J. Mol. Biol. (2003) [Pubmed]
  2. Molecular cloning and sequence of the Bacillus stearothermophilus translational initiation factor IF2 gene. Brombach, M., Gualerzi, C.O., Nakamura, Y., Pon, C.L. Mol. Gen. Genet. (1986) [Pubmed]
  3. Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli. Plumbridge, J.A., Deville, F., Sacerdot, C., Petersen, H.U., Cenatiempo, Y., Cozzone, A., Grunberg-Manago, M., Hershey, J.W. EMBO J. (1985) [Pubmed]
  4. Initiation factor IF 2 binds to the alpha-sarcin loop and helix 89 of Escherichia coli 23S ribosomal RNA. La Teana, A., Gualerzi, C.O., Dahlberg, A.E. RNA (2001) [Pubmed]
  5. Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2. Shazand, K., Tucker, J., Chiang, R., Stansmore, K., Sperling-Petersen, H.U., Grunberg-Manago, M., Rabinowitz, J.C., Leighton, T. J. Bacteriol. (1990) [Pubmed]
  6. Superexpression and fast purification of E coli initiation factor IF2. Mortensen, K.K., Nyengaard, N.R., Hershey, J.W., Laalami, S., Sperling-Petersen, H.U. Biochimie (1991) [Pubmed]
 
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